Genetic dissection of plant cell-wall biosynthesis

被引:27
|
作者
Bonetta, DT [1 ]
Facette, M [1 ]
Raab, TK [1 ]
Somerville, CR [1 ]
机构
[1] Carnegie Inst Washington, Dept Plant Biol, Stanford, CA 94305 USA
关键词
Arabidopsis; cellulose synthase; Fourer-transform IR; polysaccharide biosynthesis;
D O I
10.1042/BST0300298
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The plant cell wall is a complex structure consisting of a variety of polymers including cellulose, xyloglucan, xylan and polygalacturonan. Biochemical and genetic analysis has made it possible to clone genes encoding cellulose synthases (CesA). A comparison of the predicted protein sequences in the Arabidopsis genome indicates that 30 divergent genes with similarity to CesAs exist. It is possible that these cellulose synthase-like (Csl) proteins do not contribute to cellulose synthesis, but rather to the synthesis of other wall polymers. A major challenge is, therefore, to assign biological function to these genes. In an effort to address this issue we have systematically identified T-DNA or transposon insertions in 17 Arabidopsis Csls. Phenotypic characterization of 'knock-out' mutants includes the determination of spectroscopic profile differences in mutant cell walls from wild-type plants by Fourier-transform IR microscopy. A more precise characterization includes cell wall fractionation followed by neutral sugar composition analysis by anionic exchange chromatography.
引用
收藏
页码:298 / 301
页数:4
相关论文
共 50 条
  • [41] Substrate analogues to study cell-wall biosynthesis and its inhibition
    Lazar, K
    Walker, S
    CURRENT OPINION IN CHEMICAL BIOLOGY, 2002, 6 (06) : 786 - 793
  • [42] MURH, A NEW GENETIC-LOCUS IN ESCHERICHIA-COLI INVOLVED IN CELL-WALL PEPTIDOGLYCAN BIOSYNTHESIS
    DAI, D
    ISHIGURO, EE
    JOURNAL OF BACTERIOLOGY, 1988, 170 (05) : 2197 - 2201
  • [43] AGGLUTINATION OF PLANT PROTOPLASTS BY FUNGAL CELL-WALL GLUCANS
    PETERS, BM
    CRIBBS, DH
    STELZIG, DA
    SCIENCE, 1978, 201 (4353) : 364 - 365
  • [44] CELL-WALL TEXTURE IN GROWING-PLANT CELLS
    SASSEN, MMA
    ACTA BOTANICA NEERLANDICA, 1988, 37 (03): : 403 - 403
  • [45] HYSTERESIS OF PLANT CELL-WALL BETA-GLUCOSIDASE
    CHERON, G
    NOAT, G
    RICARD, J
    BIOCHEMICAL JOURNAL, 1990, 269 (02) : 389 - 392
  • [46] CELL-WALL OUTGROWTHS ASSOCIATED WITH INFECTION BY A PLANT RHABDOVIRUS
    DIFRANCO, A
    RUSSO, M
    MARTELLI, GP
    JOURNAL OF GENERAL VIROLOGY, 1980, 49 (JUL): : 221 - 224
  • [47] ENZYMATIC DEGRADATION OF CELL-WALL AND RELATED PLANT POLYSACCHARIDES
    WARD, OP
    MOOYOUNG, M
    CRITICAL REVIEWS IN BIOTECHNOLOGY, 1989, 8 (04) : 237 - 274
  • [48] Genetic dissection of cyclic pyranopterin monophosphate biosynthesis in plant mitochondria
    Kruse, Inga
    Maclean, Andrew E.
    Hill, Lionel
    Balk, Janneke
    BIOCHEMICAL JOURNAL, 2018, 475 : 495 - 509
  • [49] The plant cell wall: Biosynthesis, construction, and functions
    Baocai Zhang
    Yihong Gao
    Lanjun Zhang
    Yihua Zhou
    Journal of Integrative Plant Biology, 2021, 63 (01) : 251 - 272
  • [50] BIOSYNTHESIS OF PLANT-CELL WALL POLYSACCHARIDES
    DELMER, DP
    FEDERATION PROCEEDINGS, 1985, 44 (04) : 1048 - 1048