Development, Characterization and Cross Species Amplification of Polymorphic Microsatellite Markers from Expressed Sequence Tags of Turmeric (Curcuma longa L.)

被引：28
作者：
Siju, S. [1 ]
Dhanya, K. [1 ]
Syamkumar, S. [1 ]
Sasikumar, B. [1 ]
Sheeja, T. E. [1 ]
Bhat, A. I. [2 ]
Parthasarathy, V. A. [1 ]
机构：
[1] Indian Inst Spices Res, Div Crop Improvement & Biotechnol, Calicut 673012, Kerala, India
[2] Indian Inst Spices Res, Div Crop Protect, Calicut 673012, Kerala, India
来源：
MOLECULAR BIOTECHNOLOGY | 2010年 / 44卷 / 02期
关键词：
Cross species amplification; Curcuma longa L; Expressed sequence tags; Microsatellite markers; Turmeric; EST-SSR MARKERS; FREQUENCY; REPEATS; PLANTS; TRANSFERABILITY; DATABASES; BARLEY; COFFEE; RICE;
D O I：
10.1007/s12033-009-9222-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Expressed sequence tags (ESTs) from turmeric (Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average density of Class I SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of microsatellite repeat in turmeric ESTs followed by trinucleotides. A robust set of 17 polymorphic EST-SSRs were developed and used for evaluating 20 turmeric accessions. The number of alleles detected ranged from 3 to 8 per loci. The developed markers were also evaluated in 13 related species of C. longa confirming high rate (100%) of cross species transferability. The polymorphic microsatellite markers generated from this study could be used for genetic diversity analysis and resolving the taxonomic confusion prevailing in the genus.
引用
收藏
页码：140 / 147
页数：8
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