A simple one-tube assay for immunophenotypical quantification of leukemic stem cells in acute myeloid leukemia

被引：93

作者：

Zeijlemaker, W. ^{[1
]}

Kelder, A. ^{[1
]}

Oussoren-Brockhoff, Y. J. M. ^{[1
]}

Scholten, W. J. ^{[1
]}

Snel, A. N. ^{[1
]}

Veldhuizen, D. ^{[1
]}

Cloos, J. ^{[1
,2
]}

Ossenkoppele, G. J. ^{[1
]}

Schuurhuis, G. J. ^{[1
]}

机构：

[1] Vrije Univ Amsterdam, Med Ctr, Canc Ctr Amsterdam, Dept Hematol, CCA 4-24,De Boelelaan 1117, NL-1081 HV Amsterdam, Netherlands

[2] Vrije Univ Amsterdam, Med Ctr, Dept Pediat Oncol Hematol, Amsterdam, Netherlands

来源：

LEUKEMIA | 2016年 / 30卷 / 02期

关键词：

MINIMAL RESIDUAL DISEASE; INITIATING CELLS; AML; EXPRESSION; REMISSION; MARKER; MUTATIONS; DIAGNOSIS; EVOLUTION; IDENTIFICATION;

D O I：

10.1038/leu.2015.252

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Relapses after initial successful treatment in acute myeloid leukemia are thought to originate from the outgrowth of leukemic stem cells. Their flow cytometrically assessed frequency is of importance for relapse prediction and is therefore assumed to be implemented in future risk group profiling. Since current detection methods are complex, time- and bone marrow consuming (multiple-tubes approach), it would be advantageous to have a broadly applicable approach that enables to quantify leukemia stem cells both at diagnosis and follow-up. We compared 15 markers in 131 patients concerning their prevalence, usefulness and stability in CD34(+) CD38(-) leukemic stem cell detection in healthy controls, acute myeloid leukemia diagnosis and follow-up samples. Ultimately, we designed a single 8-color detection tube including common markers CD45, CD34 and CD38, and specific markers CD45RA, CD123, CD33, CD44 and a marker cocktail (CLL-1/TIM-3/CD7/CD11b/CD22/CD56) in one fluorescence channel. Validation analyses in 31 patients showed that the single tube approach was as good as the multiple-tube approach. Our approach requires the least possible amounts of bone marrow, and is suitable for multi-institutional studies. Moreover, it enables detection of leukemic stem cells both at time of diagnosis and follow-up, thereby including initially low-frequency populations emerging under therapy pressure.

引用

页码：439 / 446

页数：8

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