A PCR-based method for detection and quantification of small RNAs

被引：136
|
作者：
Ro, Seungil [1 ]
Park, Chanjae [1 ]
Jin, Jingling [1 ]
Sanders, Kenton M. [1 ]
Yan, Wei [1 ]
机构：
[1] Univ Nevada, Sch Med, Dept Physiol & Cell Biol, Reno, NV 89557 USA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 351卷 / 03期
关键词：
micro RNA (miRNA); Piwi-interacting RNA (piRNA); small nucleolar RNAs (snoRNAs); real-time quantitative PCR (Q-PCR); small RNA cDNA (srcDNA);
D O I：
10.1016/j.bbrc.2006.10.105
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Recent cloning efforts have identified hundreds of thousands of small RNAs including micro RNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs). These non-coding small RNAs need to be further validated and characterized by detecting and quantifying their expression in different tissues and during different developmental courses. A simple, accurate, and sensitive method for small RNA expression profiling is in high demand. Here, we report such a PCR-based method. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码：756 / 763
页数：8
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