Quantitative isolation of biocontrol agents Trichoderma spp., Gliocladium spp. and actinomycetes from soil with culture media

被引：94
|
作者：
Gil, S. Vargas [1 ]
Pastor, S. [2 ]
March, G. J. [1 ]
机构：
[1] IFFIVE INTA, RA-5119 Cordoba, Argentina
[2] Agrodiag SP, RA-5000 Cordoba, Argentina
来源：
MICROBIOLOGICAL RESEARCH | 2009年 / 164卷 / 02期
关键词：
Soil biodiversity; Biocontrol agents; Culture medium; Biocides; SELECTIVE MEDIUM; COMMUNITY STRUCTURE; MICROBIAL NUMBERS; DIVERSITY; RHIZOSPHERE; BACTERIAL; COLONIZATION; ENUMERATION; POPULATION; ECOLOGY;
D O I：
10.1016/j.micres.2006.11.022
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Soil biodiversity plays a key role in the sustainability of agriculture systems and indicates the level of health of soil, especially when considering the richness of microorganisms that are involved in biological. control of soilborne diseases. Cultural practices may produce changes in soil microflora, which can be quantified through the isolation of target microorganisms. Rhizosphere soil samples were taken from an assay with different crop rotations and tillage systems, and populations of Trichoderma spp., Gliocladium spp. and actinomycetes were quantified in order to select the general and selective culture media that better reflect the changes of these microbial populations in soil. The most efficient medium for the isolation of Trichoderma spp. and Gliocladium spp. was potato dextrose agar modified by the addition of chloramphenicol, streptomycin and rose bengal, and for actinomycetes was Kuster medium, with cycloheximide and sodium propionate. (C) 2007 Elsevier GmbH. All rights reserved.
引用
收藏
页码：196 / 205
页数：10
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