TaClpS1, negatively regulates wheat resistance against Puccinia striiformis f. sp. tritici

被引：8

作者：

Yang, Qian ^{[1
]}

Islam, Md Ashraful ^{[1
]}

Cai, Kunyan ^{[1
]}

Tian, Shuxin ^{[1
]}

Liu, Yan ^{[1
]}

Kang, Zhensheng ^{[1
]}

Guo, Jun ^{[1
]}

机构：

[1] Northwest A&F Univ, State Key Crop Stress Biol Arid Areas, Coll Plant Protect, Yangling 712100, Shaanxi, Peoples R China

来源：

BMC PLANT BIOLOGY | 2020年 / 20卷 / 01期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

TaClpS1; Puccinia striiformis f; sp; tritici; Wheat; Virus-induced gene silencing; Heterologous expression; PLANT-CELL DEATH; END RULE PATHWAY; STRIPE RUST; TETRAPYRROLE BIOSYNTHESIS; POSTTRANSLATIONAL CONTROL; SUBSTRATE RECOGNITION; ACQUIRED-RESISTANCE; CLP PROTEASE; ADAPTER; COMPLEX;

D O I：

10.1186/s12870-020-02762-0

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

BackgroundThe degradation of intracellular proteins plays an essential role in plant responses to stressful environments. ClpS1 and E3 ubiquitin ligase function as adaptors for selecting target substrates in caseinolytic peptidase (Clp) proteases pathways and the 26S proteasome system, respectively. Currently, the role of E3 ubiquitin ligase in the plant immune response to pathogens is well defined. However, the role of ClpS1 in the plant immune response to pathogens remains unknown.ResultsHere, wheat (Triticum aestivum) ClpS1 (TaClpS1) was studied and resulted to encode 161 amino acids, containing a conserved ClpS domain and a chloroplast transit peptide (1-32 aa). TaClpS1 was found to be specifically localized in the chloroplast when expressed transiently in wheat protoplasts. The transcript level of TaClpS1 in wheat was significantly induced during infection by Puccinia striiformis f. sp. tritici (Pst). Knockdown of TaClpS1 via virus-induced gene silencing (VIGS) resulted in an increase in wheat resistance against Pst, accompanied by an increase in the hypersensitive response (HR), accumulation of reactive oxygen species (ROS) and expression of TaPR1 and TaPR2, and a reduction in the number of haustoria, length of infection hypha and infection area of Pst. Furthermore, heterologous expression of TaClpS1 in Nicotiana benthamiana enhanced the infection by Phytophthora parasitica.ConclusionsThese results suggest that TaClpS1 negatively regulates the resistance of wheat to Pst.

引用

页数：13

共 43 条

[1] Posttranslational Control of ALA Synthesis Includes GluTR Degradation by Clp Protease and Stabilization by GluTR-Binding Protein [J].

Apitz, Janina ;

Nishimura, Kenji ;

Schmied, Judith ;

Wolf, Anja ;

Hedtke, Boris ;

van Wijk, Klaas J. ;

Grimm, Bernhard .

PLANT PHYSIOLOGY, 2016, 170 (04) :2040-2051

[2] Nonhost Resistance of Rice to Rust Pathogens [J].

Ayliffe, Michael ;

Devilla, Rosangela ;

Mago, Rohit ;

White, Rosemary ;

Talbot, Mark ;

Pryor, Anthony ;

Leung, Hei .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2011, 24 (10) :1143-1155

[3] Race Dynamics, Diversity, and Virulence Evolution in Puccinia striiformis f. sp tritici, the Causal Agent of Wheat Stripe Rust in China from 2003 to 2007 [J].

Chen, W. Q. ;

Wu, L. R. ;

Liu, T. G. ;

Xu, S. C. ;

Jin, S. L. ;

Peng, Y. L. ;

Wang, B. T. .

PLANT DISEASE, 2009, 93 (11) :1093-1101

[4] Characterization of protein kinase PsSRPKL, a novel pathogenicity factor in the wheat stripe rust fungus [J].

Cheng, Yulin ;

Wang, Xiaojie ;

Yao, Juanni ;

Voegele, Ralf T. ;

Zhang, Yanru ;

Wang, Wumei ;

Huang, Lili ;

Kang, Zhensheng .

ENVIRONMENTAL MICROBIOLOGY, 2015, 17 (08) :2601-2617

[5] Post-translational control of tetrapyrrole biosynthesis in plants, algae, and cyanobacteria [J].

Czarnecki, Olaf ;

Grimm, Bernhard .

JOURNAL OF EXPERIMENTAL BOTANY, 2012, 63 (04) :1675-1687

[6] An Arabidopsis GluTR Binding Protein Mediates Spatial Separation of 5-Aminolevulinic Acid Synthesis in Chloroplasts [J].

Czarnecki, Olaf ;

Hedtke, Boris ;

Melzer, Michael ;

Rothbart, Maxi ;

Richter, Andreas ;

Schroeter, Yvonne ;

Pfannschmidt, Thomas ;

Grimm, Bernhard .

PLANT CELL, 2011, 23 (12) :4476-4491

[7] Mammalian pro-apoptotic bax gene enhances tobacco resistance to pathogens [J].

Dong Suomeng ;

Zhang Zhengguang ;

Zheng Xiaobo ;

Wang Yuanchao .

PLANT CELL REPORTS, 2008, 27 (09) :1559-1569

[8] The bacterial N-end rule pathway: expect the unexpected [J].

Dougan, D. A. ;

Truscott, K. N. ;

Zeth, K. .

MOLECULAR MICROBIOLOGY, 2010, 76 (03) :545-558

[9] AAA plus proteins and substrate recognition, it all depends on their partner in crime [J].

Dougan, DA ;

Mogk, A ;

Zeth, K ;

Turgay, K ;

Bukau, B .

FEBS LETTERS, 2002, 529 (01) :6-10

[10] GmRAR1 and GmSGT1 Are Required for Basal, R Gene-Mediated and Systemic Acquired Resistance in Soybean [J].

Fu, Da-Qi ;

Ghabrial, Said ;

Kachroo, Aardra .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2009, 22 (01) :86-95

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