Replication of hepatitis E virus in FRhk-4 cell culture

被引：0
|
作者：
Dzagurov, GK
Kupriyanov, VV
Balayan, MS
机构：
来源：
VOPROSY VIRUSOLOGII | 1997年 / 42卷 / 02期
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中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The cytolytic form of hepatitis E virus reproduction in FRhk-4 cells is described. The cytopathic effect was observed by days 6-7 of the 25th passage in this cell culture. Reverse transcription with polymerase chain reaction were used to study virus reproduction daily for 10 days postinfection. The virus replication starts on day 4 after inoculation. The development of the cytopathic effect depended on the serum concentration in culture medium, whereas the virus replication did not depend on this factor. The replicative form (RNA) of viral RNA appeared on day 4 postinoculation. as did the new genomic one (+RNA).
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页码：63 / 66
页数：4
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