Vaccination with dendritic cells pulsed with homogenate protein of spinal cord promotes functional recovery from spinal cord injury in mice

被引:12
作者
Liu, M. [1 ]
Zhao, J. [1 ]
Liang, H. [2 ]
Bian, X. [3 ]
机构
[1] Third Mil Med Univ, Daping Hosp, Dept Orthopaed, Chongqing 400042, Peoples R China
[2] Third Mil Med Univ, Inst Surg Res, State Key Lab Trauma Burn & Combined Injury, Chongqing 400042, Peoples R China
[3] Third Mil Med Univ, Southwest Hosp, Inst Pathol, Chongqing 400042, Peoples R China
关键词
spinal cord injury; dendritic cells; homogenate protein of spinal cord; AUTOIMMUNE T-CELLS; RAT; MACROPHAGES;
D O I
10.1038/sc.2008.112
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Study design: Randomized, double-blinded animal experiment for neural functional recovery from spinal cord injury (SCI) through vaccination with immature dendritic cells (DCs) pulsed with homogenate protein of spinal cord (hpDCs) in mice. Objective: To study the effect of hpDCs in the recovery from SCI in mice. Method: Immature DCs pulsed with homogenate protein of spinal cord, myelin basic protein (MBP) or phosphate-buffer solution (PBS) were injected into spinal cord-injured mice locally or peritoneally. The functional recovery of spinal cord (open-field locomotor rating scale of Basso, Beattie and Bresnahan, BBB score) was measured weekly. The areas of injured region and cyst as well as the thickness of the glial scar were measured and the expressions of glial fibrillary acidic protein, neurofilament and nestin were detected to confirm the BBB scores. Results: Eighty-four days after injection, the BBB score of the hpDCs group (peritoneally injected mice) reached 18.2 +/- 1.1, significantly higher than that the scores of the mbpDCs and control groups (16.3 +/- 2.1 and 10.0 +/- 2.0, respectively). The areas of injured region and cyst as well as the thickness of the glial scar of the hpDCs group were less than that of the control group. Meanwhile, the expression of nestin lasts up to 56 days after injection in the hpDCs group, while it disappeared in the mbpDCs and PBS groups. Conclusion: Implanting DCs pulsed with homogenate protein of spinal cord, but not mbpDCs or PBS alone, locally or peritoneally, have a significant effect on functional recovery and neural preservation from SCI. Spinal Cord (2009) 47, 360-366; doi:10.1038/sc.2008.112; published online 30 September 2008
引用
收藏
页码:360 / 366
页数:7
相关论文
共 50 条
[21]   Optogenetic Neuronal Stimulation Promotes Functional Recovery After Spinal Cord Injury [J].
Deng, Wei-wei ;
Wu, Guang-yan ;
Min, Ling-xia ;
Feng, Zhou ;
Chen, Hui ;
Tan, Ming-liang ;
Sui, Jian-feng ;
Liu, Hong-liang ;
Hou, Jing-ming .
FRONTIERS IN NEUROSCIENCE, 2021, 15
[22]   Proanthocyanidin promotes functional recovery of spinal cord injury via inhibiting ferroptosis [J].
Zhou Huangao ;
Yin Chaoyun ;
Zhang Zhiming ;
Tang Haowen ;
Shen Wen ;
Zha Xuan ;
Gao Ming ;
Sun Jifu ;
Xu Xiuquan ;
Chen Qian .
JOURNAL OF CHEMICAL NEUROANATOMY, 2020, 107
[23]   Acute baclofen administration promotes functional recovery after spinal cord injury [J].
Pinho, Andreia G. ;
Monteiro, Susana ;
Liberato, Valentina ;
Santos, Diogo J. ;
Campos, Jonas ;
Cibra, Jorge R. ;
Silva, Nuno A. ;
de Sousa, Nidia ;
Barreiro-Iglesias, Anton ;
Salgado, Antonio J. .
SPINE JOURNAL, 2023, 23 (03) :379-391
[24]   A CD1D DEFICIENCY IN MICE PROMOTES FUNCTIONAL RECOVERY AFTER A SPINAL CORD INJURY [J].
Wu, Xiangbing ;
Liu, Jianyun ;
Medina, Laura Yunes ;
Yaacoub, Alen ;
Khan, Mohammad Faizan ;
Brutkiewicz, Randy ;
Xu, Xiao-Ming .
JOURNAL OF NEUROTRAUMA, 2019, 36 (13) :A87-A88
[25]   CXCL12 promotes spinal nerve regeneration and functional recovery after spinal cord injury [J].
Li, Jingyuan ;
Wu, Yanqiong ;
Chen, Ping ;
Huang, Xiaoxia ;
Liu, Yongli ;
Peng, Minjing ;
Wu, Rong-Qian .
NEUROREPORT, 2021, 32 (06) :450-457
[26]   Galectin-3 inhibition reduces fibrotic scarring and promotes functional recovery after spinal cord injury in mice [J].
Shan, Fangli ;
Ye, Jianan ;
Xu, Xinzhong ;
Liang, Chao ;
Zhao, Yuanzhe ;
Wang, Jingwen ;
Ouyang, Fangru ;
Li, Jianjian ;
Lv, Jianwei ;
Wu, Zhonghan ;
Yao, Fei ;
Jing, Juehua ;
Zheng, Meige .
CELL AND BIOSCIENCE, 2024, 14 (01)
[27]   A polysialic acid mimetic peptide promotes functional recovery in a mouse model of spinal cord injury [J].
Marino, Philippe ;
Norreel, Jean-Chretien ;
Schachner, Melitta ;
Rougon, Genevieve ;
Amoureux, Marie-Claude .
EXPERIMENTAL NEUROLOGY, 2009, 219 (01) :163-174
[28]   Endocrine Therapy for the Functional Recovery of Spinal Cord Injury [J].
Wang, Hui ;
Zhou, Wen-xian ;
Huang, Jin-feng ;
Zheng, Xuan-qi ;
Tian, Hai-jun ;
Wang, Bin ;
Fu, Wei-li ;
Wu, Ai-min .
FRONTIERS IN NEUROSCIENCE, 2020, 14
[29]   FTY720 Reduces Inflammation and Promotes Functional Recovery after Spinal Cord Injury [J].
Lee, Kangmin D. ;
Chow, Woon N. ;
Sato-Bigbee, Carmen ;
Graf, Martin R. ;
Graham, Robert S. ;
Colello, Raymond J. ;
Young, Harold F. ;
Mathern, Bruce E. .
JOURNAL OF NEUROTRAUMA, 2009, 26 (12) :2335-2344
[30]   Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in Wistar rats [J].
Rodrigues, L. P. ;
Iglesias, D. ;
Nicola, F. C. ;
Steffens, D. ;
Valentim, L. ;
Witczak, A. ;
Zanatta, G. ;
Achaval, M. ;
Pranke, P. ;
Netto, C. A. .
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, 2012, 45 (01) :49-57