Evaluation of a new generation of culture bottle using an automated bacterial culture system for detecting nine common contaminating organisms found in platelet components

被引:48
作者
Brecher, ME
Heath, DG
Hay, SN
Rothenberg, SJ
Stutzman, LC
机构
[1] Univ N Carolina, Dept Pathol & Lab Med, Transfus Med Serv, Chapel Hill, NC USA
[2] Univ N Carolina, Dept Clin Microbiol, Chapel Hill, NC USA
[3] Organon Teknika, BioMerieux, Durham, NC USA
关键词
D O I
10.1046/j.1537-2995.2002.00122.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
BACKGROUND: An automated bacterial culture system (BacT/ALERT 3D, bioMerieux) has been previously validated with a variety of bacteria in platelets. The recovery of bacteria in platelets using a new generation of culture bottles that do not require venting and that use a liquid emulsion sensor was studied. STUDY DESIGN AND METHODS: Bacillus cereus, Enterobacter cloacae, Escherichia coli, Klebsiella oxytoca, Staphylococcus aureus, Staphylococcus epidermidis, Serratia marcescens, Streptococcus viridans, and Propionibacterium acnes isolates were inoculated into Day 2 platelets to concentrations of 10 and 100 CFU per mL. Samples were then studied with current and new aerobic, anaerobic, and pediatric bottles. RESULTS: All organisms, except P. acnes, were detected in a mean time of 9.2 to 20.4 (10 CFU/mL) or 8.7 to 18.6 (100 CFU/mL) hours. P. acnes was detected in a mean time of 69.2 (10 CFU/mL) or 66.0 (100 CFU/ mL) hours. The 10-fold increase in inoculum was associated with a mean 9.2 percent difference in detection time. The aerobic, anaerobic, and pediatric bottles had a mean difference in detection time (hours) between the current and new bottles of 0.10 (p = 0.61), 0.4 (p 0.38), and 1.0 (p < 0.001), respectively. CONCLUSION: No difference in detection time between the current and new aerobic and anaerobic bottles was demonstrated. The new pediatric bottles had a small but significant delay in detection.
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页码:774 / 779
页数:6
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