Characterization of soluble non-covalent complexes between bovine serum albumin and β-1,2,3,4,6-penta-O-galloyl-D-glucopyranose by MALDI-TOF MS

被引：70
作者：
Chen, YM [1 ]
Hagerman, AE [1 ]
机构：
[1] Miami Univ, Dept Chem & Biochem, Oxford, OH 45056 USA
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2004年 / 52卷 / 12期
关键词：
hydrolyzable tannin; polyphenolic; protein -tannin interaction; pentagalloyl glucose; MALDI-TOF MS;
D O I：
10.1021/jf035536t
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
beta-1,2,3,4,6-Penta-O-galloyl-D-glucopyranose (PGG) and soluble complexes of PGG with bovine serum albumin (BSA) were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). PGG was also characterized by electrospray ionization mass spectrometry (ESI-MS). Similar fragmentation patterns of PGG were found in ESI-MS and MALDI-TOF MS. The apparent stoichiometries of non-covalent BSA-PGG complexes were determined by MALDI-TOF MS.
引用
收藏
页码：4008 / 4011
页数：4
相关论文
共 30 条
  • [1] Multiple interactions between polyphenols and a salivary proline-rich protein repeat result in complexation and precipitation
    Baxter, NJ
    Lilley, TH
    Haslam, E
    Williamson, MP
    [J]. BIOCHEMISTRY, 1997, 36 (18) : 5566 - 5577
  • [2] Chemical studies of proanthocyanidins and hydrolyzable tannins
    Bors, W
    Foo, LY
    Hertkorn, N
    Michel, C
    Stettmaier, K
    [J]. ANTIOXIDANTS & REDOX SIGNALING, 2001, 3 (06) : 995 - 1008
  • [3] Polyphenol/peptide binding and precipitation
    Charlton, AJ
    Baxter, NJ
    Khan, ML
    Moir, AJG
    Haslam, E
    Davies, AP
    Williamson, MP
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (06) : 1593 - 1601
  • [4] Binding affinities of gallotannin analogs with bovine serum albumin: ramifications for polyphenol-protein molecular recognition
    Feldman, KS
    Sambandam, A
    Lemon, ST
    Nicewonger, RB
    Long, GS
    Battaglia, DF
    Ensel, SM
    Laci, MA
    [J]. PHYTOCHEMISTRY, 1999, 51 (07) : 867 - 872
  • [5] Probing protein-tannin interactions by isothermal titration microcalorimetry
    Frazier, RA
    Papadopoulou, A
    Mueller-Harvey, I
    Kissoon, D
    Green, RJ
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (18) : 5189 - 5195
  • [6] Glocker MO, 1996, J MASS SPECTROM, V31, P1221, DOI 10.1002/(SICI)1096-9888(199611)31:11<1221::AID-JMS410>3.0.CO
  • [7] 2-P
  • [8] Metabolism of dietary procyanidins in rats
    Gonthier, MP
    Donovan, JL
    Texier, O
    Felgines, C
    Remesy, C
    Scalbert, A
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 2003, 35 (08) : 837 - 844
  • [9] High molecular weight plant polyphenolics (tannins) as biological antioxidants
    Hagerman, AE
    Riedl, KM
    Jones, GA
    Sovik, KN
    Ritchard, NT
    Hartzfeld, PW
    Riechel, TL
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1998, 46 (05) : 1887 - 1892
  • [10] Methods for determination of condensed and hydrolyzable tannins
    Hagerman, AE
    Zhao, Y
    Johnson, S
    [J]. ANTINUTRIENTS AND PHYTOCHEMICALS IN FOOD, 1997, 662 : 209 - 222
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