Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn2+ Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots

被引:119
作者
Ahmed, Bilal [1 ]
Dwivedi, Sourabh [1 ,2 ]
Abdin, Malik Zainul [3 ]
Azam, Ameer [2 ]
Al-Shaeri, Majed [4 ]
Khan, Mohammad Saghir [1 ]
Saquib, Quaiser [5 ]
Al-Khedhairy, Abdulaziz A. [5 ]
Musarrat, Javed [1 ,6 ]
机构
[1] Aligarh Muslim Univ, Fac Agr Sci, Dept Agr Microbiol, Aligarh 202002, Uttar Pradesh, India
[2] Aligarh Muslim Univ, Dept Appl Phys, Aligarh 202002, Uttar Pradesh, India
[3] Jamia Hamdard, Dept Biotechnol, New Delhi 110062, India
[4] King Abdulaziz Univ, Dept Biol Sci, Fac Sci, Jeddah, Saudi Arabia
[5] King Saud Univ, Dept Zool, Coll Sci, POB 2455, Riyadh 11451, Saudi Arabia
[6] Baba Ghulam Shah Badshah Univ, Sch Biosci & Biodivers, Rajouri, J&K, India
关键词
COMPARATIVE PHYTOTOXICITY; INFRARED-SPECTROSCOPY; SILVER NANOPARTICLES; OXIDE NANOPARTICLES; ZINC-OXIDE; CELL-DEATH; L; BIOSYNTHESIS; CYTOTOXICITY; TOXICITY;
D O I
10.1038/srep40685
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Large-scale synthesis and release of nanomaterials in environment is a growing concern for human health and ecosystem. Therefore, we have investigated the cytotoxic and genotoxic potential of zinc oxide nanoparticles (ZnO-NPs), zinc oxide bulk (ZnO-Bulk), and zinc ions (Zn2+) in treated roots of Allium cepa, under hydroponic conditions. ZnO-NPs were characterized by UV-visible, XRD, FT-IR spectroscopy and TEM analyses. Bulbs of A. cepa exposed to ZnO-NPs (25.5 nm) for 12 h exhibited significant decrease (23 +/- 8.7%) in % mitotic index and increase in chromosomal aberrations (18 +/- 7.6%), in a dose-dependent manner. Transmission electron microcopy and FT-IR data suggested surface attachment, internalization and biomolecular intervention of ZnO-NPs in root cells, respectively. The levels of TBARS and antioxidant enzymes were found to be significantly greater in treated root cells vis-a-vis untreated control. Furthermore, dose-dependent increase in ROS production and alterations in Delta Psi m were observed in treated roots. FT-IR analysis of root tissues demonstrated symmetric and asymmetric P=O stretching of >PO2- at 1240 cm(-1) and stretching of C-O ribose at 1060 cm(-1), suggestive of nuclear damage. Overall, the results elucidated A. cepa, as a good model for assessment of cytotoxicity and oxidative DNA damage with ZnO-NPs and Zn2+ in plants.
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页数:14
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