Ratio of intracellular precursors concentration and their flux influences hyaluronic acid molecular weight in Streptococcus zooepidemicus and recombinant Lactococcus lactis

被引：44

作者：

Badle, Sneh Sanjay ^{[1
]}

Jayaraman, Guhan ^{[1
]}

Ramachandran, K. B. ^{[1
]}

机构：

[1] Indian Inst Technol, Bhupat & Jyoti Mehta Sch Biosci, Dept Biotechnol, Madras 600036, Tamil Nadu, India

来源：

BIORESOURCE TECHNOLOGY | 2014年 / 163卷

关键词：

Hyaluronic acid; Molecular weight; MFA; Lactococcus lactis; Streptococcus zooepidemicus; BACILLUS-SUBTILIS; BIOSYNTHESIS; GENES; COLI;

D O I：

10.1016/j.biortech.2014.04.027

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

HA molecular weight variation in Streptococcus zooepidemicus and two recombinant Lactococcus lactis strains were investigated by chemostat experiments and metabolic flux analysis (MFA). The study showed that intracellular flux ratio of UDP-GlcUA to UDP-GIcNAc correlated directly with HA molecular weight, for all the three strains. The ratio of intracellular concentration of these HA precursors also exhibited a similar trend. Phosphoglucoisomerase activity and glucose flux towards lactic acid formation were found to be the major bottlenecks for HA production in all the three strains. The study suggests that environmental conditions and genetic manipulations that balance the intracellular flux and HA precursors concentrations will result in increased molecular weight. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：222 / 227

页数：6

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