Ratio of intracellular precursors concentration and their flux influences hyaluronic acid molecular weight in Streptococcus zooepidemicus and recombinant Lactococcus lactis

被引：42
|
作者：
Badle, Sneh Sanjay [1 ]
Jayaraman, Guhan [1 ]
Ramachandran, K. B. [1 ]
机构：
[1] Indian Inst Technol, Bhupat & Jyoti Mehta Sch Biosci, Dept Biotechnol, Madras 600036, Tamil Nadu, India
来源：
BIORESOURCE TECHNOLOGY | 2014年 / 163卷
关键词：
Hyaluronic acid; Molecular weight; MFA; Lactococcus lactis; Streptococcus zooepidemicus; BACILLUS-SUBTILIS; BIOSYNTHESIS; GENES; COLI;
D O I：
10.1016/j.biortech.2014.04.027
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
HA molecular weight variation in Streptococcus zooepidemicus and two recombinant Lactococcus lactis strains were investigated by chemostat experiments and metabolic flux analysis (MFA). The study showed that intracellular flux ratio of UDP-GlcUA to UDP-GIcNAc correlated directly with HA molecular weight, for all the three strains. The ratio of intracellular concentration of these HA precursors also exhibited a similar trend. Phosphoglucoisomerase activity and glucose flux towards lactic acid formation were found to be the major bottlenecks for HA production in all the three strains. The study suggests that environmental conditions and genetic manipulations that balance the intracellular flux and HA precursors concentrations will result in increased molecular weight. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：222 / 227
页数：6
相关论文
共 26 条
  • [1] Production of controlled molecular weight hyaluronic acid by glucostat strategy using recombinant Lactococcus lactis cultures
    Jeeva, Pandeeswari
    Doss, Sreeja Shanmuga
    Sundaram, Vijayakumar
    Jayaraman, Guhan
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2019, 103 (11) : 4363 - 4375
  • [2] Chromosomal integration of hyaluronic acid synthesis (has) genes enhances the molecular weight of hyaluronan produced in Lactococcus lactis
    Hmar, Rothangmawi Victoria
    Prasad, Shashi Bala
    Jayaraman, Guhan
    Ramachandran, Kadathur B.
    BIOTECHNOLOGY JOURNAL, 2014, 9 (12) : 1554 - 1564
  • [3] Enhancement of acetyl-CoA by acetate co-utilization in recombinant Lactococcus lactis cultures enables the production of high molecular weight hyaluronic acid
    Puvendran, Kirubhakaran
    Jayaraman, Guhan
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2019, 103 (17) : 6989 - 7001
  • [4] The Role of Hyaluronic Acid Precursor Concentrations in Molecular Weight Control in Streptococcus zooepidemicus
    Chen, Wendy Yiting
    Marcellin, Esteban
    Steen, Jennifer A.
    Nielsen, Lars Keld
    MOLECULAR BIOTECHNOLOGY, 2014, 56 (02) : 147 - 156
  • [5] Understanding the Influence of Phosphatidylcholine on the Molecular Weight of Hyaluronic Acid Synthesized by Streptococcus zooepidemicus
    Sun, Jingfeng
    Wang, Miaomiao
    Chen, Yanrong
    Shang, Fei
    Ye, Hua
    Tan, Tianwei
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2012, 168 (01) : 47 - 57
  • [6] Understanding the Influence of Phosphatidylcholine on the Molecular Weight of Hyaluronic Acid Synthesized by Streptococcus zooepidemicus
    Jingfeng Sun
    Miaomiao Wang
    Yanrong Chen
    Fei Shang
    Hua Ye
    Tianwei Tan
    Applied Biochemistry and Biotechnology, 2012, 168 : 47 - 57
  • [7] Improved Yield of High Molecular Weight Hyaluronic Acid Production in a Stable Strain of Streptococcus zooepidemicus via the Elimination of the Hyaluronidase-Encoding Gene
    Pourzardosht, Navid
    Rasaee, Mohammad Javad
    MOLECULAR BIOTECHNOLOGY, 2017, 59 (06) : 192 - 199
  • [8] Mechanism for the effect of agitation on the molecular weight of hyaluronic acid produced by Streptococcus zooepidemicus
    Zhang, Xu
    Duan, Xu-Jie
    Tan, Wen-Song
    FOOD CHEMISTRY, 2010, 119 (04) : 1643 - 1646
  • [9] Effect of cysteine transport on the molecular weight and synthesis of hyaluronic acid in Streptococcus zooepidemicus
    Di, Jingyi
    Pei, Xujuan
    Hu, Shaoru
    Zuo, Meng
    Liu, Hao
    Gao, Weixia
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2025, 306
  • [10] Understanding plasmid effect on hyaluronic acid molecular weight produced by Streptococcus equi subsp zooepidemicus
    Marcellin, Esteban
    Chen, Wendy Y.
    Nielsen, Lars K.
    METABOLIC ENGINEERING, 2010, 12 (01) : 62 - 69
123