Optimal amphiphile concentration for lysozyme extraction using reverse micelles

被引：0
作者：
Naoe, K [1 ]
Imai, M [1 ]
Shimizu, M [1 ]
机构：
[1] TOKYO UNIV AGR & TECHNOL, DEPT CHEM ENGN, TOKYO, JAPAN
来源：
FOOD AND BIOPRODUCTS PROCESSING | 1996年 / 74卷 / C3期
关键词：
reverse micelle; extraction; protein; lysozyme; amphiphile; AOT;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The optimal AOT concentration for the extraction of lysozyme using reverse micelles was investigated, focusing on both the amount recovered and the activity of the enzyme. At a lysozyme feed concentration of 5.0 x 10(-4) M, the total percentage extraction (E) reached a maximum at ca. 80 nm AOT. The lysozyme relative specific activity (RSA) decreased at AOT concentrations higher than 100 mM, and the optimal AOT concentration for maximum recovery of activity (E x RSA) was found to be 80 mM AOT. Recovery of activity was almost 100% at protein concentrations less than 1.0 x 10(-4) M and CD measurements showed that the lysozyme molecular conformation after the extraction was well preserved.
引用
收藏
页码：163 / 170
页数：8
相关论文
共 50 条
  • [21] Mass-transfer of lysozyme across a flat surface with reverse micelles
    Chun, BS
    Wilkinson, GT
    SEPARATION SCIENCE AND TECHNOLOGY, 2002, 37 (14) : 3323 - 3334
  • [22] One-step separation of lysozyme by reverse micelles formed by the cationic surfactant, cetyldimethylammonium bromide
    Noh, KH
    Imm, JY
    FOOD CHEMISTRY, 2005, 93 (01) : 95 - 101
  • [23] Extraction of proteins and polymers using reverse micelles and percolation process
    Dong-Pyo Hong
    Ryoichi Kuboi
    Isao Komasawa
    Korean Journal of Chemical Engineering, 1997, 14 : 334 - 340
  • [24] Study development on extraction of protein in reverse micelles technology
    Zhao Xiaoyan
    Chen Fusheng
    Xue Wentong
    Li Lite
    Innovation and Development of Urban Agricultural Engineering, 2005, : 449 - 456
  • [25] Extraction of aminoglycoside antibiotics with reverse micelles
    Hu, ZY
    Gulari, E
    JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 1996, 65 (01) : 45 - 48
  • [26] Reverse micellar extraction of lysozyme using two dialkyl sodium phosphinates
    Shin, YO
    Vera, JH
    CANADIAN JOURNAL OF CHEMICAL ENGINEERING, 2004, 82 (02) : 349 - 357
  • [27] Oxidative refolding of reduced, denatured lysozyme in AOT reverse micelles
    Fan, Jun-Bao
    Chen, Jie
    Liang, Yi
    JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2008, 322 (01) : 95 - 103
  • [28] Refolding of denatured lysozyme assisted by artificial chaperones in reverse micelles
    Dong, Xiao-Yan
    Wu, Xiao-Yue
    Sun, Yan
    BIOCHEMICAL ENGINEERING JOURNAL, 2006, 31 (01) : 92 - 95
  • [29] Effect of Parameter on Forward Extraction Amoxicillin by using Mixed Reverse Micelles
    Ahmad, Akil
    Khatoon, Asma
    Mohd-Setapar, Siti Hamidah
    Mohamad-Aziz, Siti Norazimah
    Ahmad-Zaini, Muhammad Abbas
    Chuong, Chuo Sing
    RESEARCH JOURNAL OF BIOTECHNOLOGY, 2013, 8 (10): : 10 - 14
  • [30] Extraction of Monoclonal Antibodies (IgG1) Using Anionic and Anionic/Nonionic Reverse Micelles
    George, Daliya A.
    Stuckey, David C.
    BIOTECHNOLOGY PROGRESS, 2010, 26 (05) : 1352 - 1360
12345