Seedless mechanism of a new mandarin cultivar 'Wuzishatangju' (Citrus reticulata Blanco)

被引：77

作者：

Ye, Weijia ^{[1
]}

Qin, Yonghua ^{[1
]}

Ye, Zixing ^{[1
]}

Teixeira da Silva, Jaime A. ^{[2
,3
]}

Zhang, Lingxiao ^{[4
]}

Wu, Xiaoying ^{[1
]}

Lin, Shunquan ^{[1
]}

Hu, Guibing ^{[1
]}

机构：

[1] S China Agr Univ, Coll Hort, Guangzhou 510642, Guangdong, Peoples R China

[2] Kagawa Univ, Fac Agr, Kagawa 7610795, Japan

[3] Kagawa Univ, Grad Sch Agr, Kagawa 7610795, Japan

[4] Mississippi State Univ, Delta Res & Extens Ctr, Stoneville, MS 38776 USA

来源：

PLANT SCIENCE | 2009年 / 177卷 / 01期

关键词：

Citrus reticulata Blanco. cv. Wuzishatangju; Embryo sac; Embryonic development; Male gamete; Seedless mechanism; Self-incompatibility; SELF-INCOMPATIBILITY; TRIPLOID POMELO; POLLEN; PLANTS; STERILITY; HYBRID;

D O I：

10.1016/j.plantsci.2009.03.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Seedlessness is often a desired characteristic for many types of fruits. A new seedless mandarin cultivar 'Wuzishatangju' and its original seeded cultivar 'Shatangju' were used to study the mechanism of seedlessness from the beginning of fertility. Embryonic development, compatibility of self-pollinated 'Wuzishatangju' and cross-pollinated 'Wuzishatangju' ('Wuzishatangju' x 'Ponkan', 'Wuzishatangju' x 'Shatangju') were studied. Results indicated that the male gametes of 'Wuzishatangju' were healthy. Fertility of the 'Wuzishatangju' embryo sac was normal and the mature embryo sac had one egg, two synergids, three antipodal cells and one big central cell containing two polar nuclei. Embryonic development of cross-pollinated 'Wuzishatangju' was normal. Many globular embryos and a few heart-shaped embryos were observed 2 weeks after cross-pollination (WACP). Heart- and torpedo-shaped embryos were observed at three WACP while embryos reached the torpedo stage at four WACP. Cotyledonary embryos were observed at five WACP and they developed into seeds while funiculi were still existent at seven WACP. However, embryonic development of self-pollinated 'Wuzishatangju' was abnormal and most embryos, which degenerated by 2 weeks after self-pollination (WASP), disappeared and seedless fruits were produced by four WASP. Pollen tubes of cross-pollinated 'Wuzishatangju' could grow normally in the stigma, style and ovary, and they entered into the embryo sac resulting in successful fertilization. The pollen tubes of self-pollinated 'Wuzishatangju' grew well in the stigma and style; however, when they entered into the ovary, they became twisted and could not enter the embryo sac. Results indicate that the activities of the male gamete and the fertility of the embryo sac of 'Wuzishatangiu' were functioning normally with no embryo abortion during embryonic development. Gametophytic self-incompatibility (SI) caused seedlessness in 'Wuzishatangju' by blocking fertilization in the ovary. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：19 / 27

页数：9

共 64 条

[21] Identification and evaluation of diplogyny in clementine (Citrus clementina) for use in breeding [J].

Luro, F ;

Maddy, F ;

Jacquemond, C ;

Froelicher, Y ;

Morillon, R ;

Rist, D ;

Ollitrault, P .

Proceedings of the XIth Eucarpia Symposium on Fruit Breeding and Genetics, Vols 1 and 2, 2004, (663) :841-847

[22]

LV LX, 1997, J FUJIAN AGR U, V26, P397

[23] SELF-INCOMPATIBILITY - HOW PLANTS AVOID ILLEGITIMATE OFFSPRING [J].

MATTON, DP ;

NASS, N ;

CLARKE, AE ;

NEWBIGIN, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (06) :1992-1997

[24] Molecular recognition and response in pollen and pistil interactions [J].

McCubbin, AG ;

Kao, TH .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2000, 16 :333-364

[25] A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].

MURASHIGE, T ;

SKOOG, F .

PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497

[26] USE OF POLLEN FROM A SOMATIC HYBRID BETWEEN CITRUS AND PONCIRUS IN THE PRODUCTION OF TRIPLOIDS [J].

OIYAMA, I ;

KOBAYASHI, S ;

YOSHINAGA, K ;

OHGAWARA, T ;

ISHII, S .

HORTSCIENCE, 1991, 26 (08) :1082-1082

[27]

[齐洁 Qi Jie], 2002, [山东农业大学学报. 自然科学版, Journal of shandong agricultural university], V33, P248

[28]

QIAO YG, 2002, NO HORTIC, V2, P7

[29] Learning from rejection: The evolutionary biology of single-locus incompatibility [J].

Richman, AD ;

Kohn, JR .

TRENDS IN ECOLOGY & EVOLUTION, 1996, 11 (12) :497-502

[30]

Shen DX, 1997, FRUIT BREEDING

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