Calcium signaling has long been associated with key events of immunity, including chemotaxis, phagocytosis, and activation. However, imaging and manipulation of calcium flux in motile immune cells in live animals remain challenging. Using light-sheet microscopy for in vivo calcium imaging in zebrafish, we observe characteristic patterns of calcium flux triggered by distinct events, including phagocytosis of pathogenic bacteria and migration of neutrophils toward inflammatory stimuli. In contrast to findings from ex vivo studies, we observe enriched calcium influx at the leading edge of migrating neutrophils. To directly manipulate calcium dynamics in vivo, we have developed transgenic lines with cell-specific expression of the mammalian TRPV1 channel, enabling ligand-gated, reversible, and spatiotemporal control of calcium influx. We find that controlled calcium influx can function to help define the neutrophil's leading edge. Cell-specific TRPV1 expression may have broad utility for precise control of calcium dynamics in other immune cell types and organisms.
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页码:2107 / 2117
页数:11
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[1]
Ahrens MB, 2013, NAT METHODS, V10, P413, DOI [10.1038/NMETH.2434, 10.1038/nmeth.2434]
机构:
Univ Washington, Dept Pediat, Seattle, WA 98195 USAUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Brannon, Mark K.
;
Davis, J. Muse
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机构:
Emory Univ, Immunol & Mol Pathogenesis Program, Atlanta, GA 30322 USAUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Davis, J. Muse
;
Mathias, Jonathan R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USAUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Mathias, Jonathan R.
;
Hall, Chris J.
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h-index: 0
机构:
Univ Auckland, Sch Med Sci, Dept Mol Med & Pathol, Auckland 1, New ZealandUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Hall, Chris J.
;
Emerson, Julia C.
论文数: 0引用数: 0
h-index: 0
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Univ Washington, Dept Pediat, Seattle, WA 98195 USAUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Emerson, Julia C.
;
Crosier, Philip S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Auckland, Sch Med Sci, Dept Mol Med & Pathol, Auckland 1, New ZealandUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Crosier, Philip S.
;
Huttenlocher, Anna
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Univ Wisconsin, Dept Pediat, Madison, WI USAUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Huttenlocher, Anna
;
Ramakrishnan, Lalita
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Microbiol, Seattle, WA 98195 USA
Univ Washington, Dept Med, Seattle, WA USA
Univ Washington, Dept Immunol, Seattle, WA 98195 USAUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Ramakrishnan, Lalita
;
Moskowitz, Samuel M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Pediat, Seattle, WA 98195 USAUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
机构:
Univ Washington, Dept Immunol, Seattle, WA 98195 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
Cambier, C. J.
;
Takaki, Kevin K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Microbiol, Seattle, WA 98195 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
Takaki, Kevin K.
;
Larson, Ryan P.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Immunol, Seattle, WA 98195 USA
Seattle Biomed Res Inst, Seattle, WA 98109 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
Larson, Ryan P.
;
Hernandez, Rafael E.
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h-index: 0
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Univ Washington, Dept Pediat, Seattle, WA 98195 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
Hernandez, Rafael E.
;
Tobin, David M.
论文数: 0引用数: 0
h-index: 0
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Univ Washington, Dept Microbiol, Seattle, WA 98195 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
Tobin, David M.
;
Urdahl, Kevin B.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Immunol, Seattle, WA 98195 USA
Seattle Biomed Res Inst, Seattle, WA 98109 USA
Univ Washington, Dept Pediat, Seattle, WA 98195 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
Urdahl, Kevin B.
;
Cosma, Christine L.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Microbiol, Seattle, WA 98195 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
Cosma, Christine L.
;
Ramakrishnan, Lalita
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Immunol, Seattle, WA 98195 USA
Univ Washington, Dept Microbiol, Seattle, WA 98195 USA
Univ Washington, Dept Med, Seattle, WA 98195 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
机构:
Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
Broad Inst MIT & Harvard, Cambridge, MA 02142 USAMassachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Clatworthy, Anne E.
;
Lee, Jenny See-Wai
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h-index: 0
机构:
Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
Broad Inst MIT & Harvard, Cambridge, MA 02142 USAMassachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Lee, Jenny See-Wai
;
Leibman, Mark
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h-index: 0
机构:
Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
Broad Inst MIT & Harvard, Cambridge, MA 02142 USAMassachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Leibman, Mark
;
Kostun, Zachary
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h-index: 0
机构:
Massachusetts Gen Hosp, Ctr Regenerat Med, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Med, Boston, MA 02114 USAMassachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Kostun, Zachary
;
Davidson, Alan J.
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h-index: 0
机构:
Massachusetts Gen Hosp, Ctr Regenerat Med, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Med, Boston, MA 02114 USAMassachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Davidson, Alan J.
;
Hung, Deborah T.
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h-index: 0
机构:
Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
Broad Inst MIT & Harvard, Cambridge, MA 02142 USA
Harvard Univ, Sch Med, Dept Med, Boston, MA 02114 USAMassachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
机构:
Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Univ Wisconsin, Dept Pediat, Madison, WI 53706 USAUniv Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Deng, Qing
;
Sarris, Milka
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h-index: 0
机构:
Inst Pasteur, Macrophages & Dev Immun Unit, Dept Dev & Stem Cell Biol, Paris, France
CNRS, URA2578, Paris, FranceUniv Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Sarris, Milka
;
Bennin, David A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Univ Wisconsin, Dept Pediat, Madison, WI 53706 USAUniv Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Bennin, David A.
;
Green, Julie M.
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h-index: 0
机构:
Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Univ Wisconsin, Dept Pediat, Madison, WI 53706 USAUniv Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Green, Julie M.
;
Herbomel, Philippe
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h-index: 0
机构:
Inst Pasteur, Macrophages & Dev Immun Unit, Dept Dev & Stem Cell Biol, Paris, France
CNRS, URA2578, Paris, FranceUniv Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Herbomel, Philippe
;
Huttenlocher, Anna
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Univ Wisconsin, Dept Pediat, Madison, WI 53706 USAUniv Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
机构:
Univ Washington, Dept Pediat, Seattle, WA 98195 USAUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Brannon, Mark K.
;
Davis, J. Muse
论文数: 0引用数: 0
h-index: 0
机构:
Emory Univ, Immunol & Mol Pathogenesis Program, Atlanta, GA 30322 USAUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Davis, J. Muse
;
Mathias, Jonathan R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USAUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Mathias, Jonathan R.
;
Hall, Chris J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Auckland, Sch Med Sci, Dept Mol Med & Pathol, Auckland 1, New ZealandUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Hall, Chris J.
;
Emerson, Julia C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Pediat, Seattle, WA 98195 USAUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Emerson, Julia C.
;
Crosier, Philip S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Auckland, Sch Med Sci, Dept Mol Med & Pathol, Auckland 1, New ZealandUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Crosier, Philip S.
;
Huttenlocher, Anna
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Univ Wisconsin, Dept Pediat, Madison, WI USAUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Huttenlocher, Anna
;
Ramakrishnan, Lalita
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Microbiol, Seattle, WA 98195 USA
Univ Washington, Dept Med, Seattle, WA USA
Univ Washington, Dept Immunol, Seattle, WA 98195 USAUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
Ramakrishnan, Lalita
;
Moskowitz, Samuel M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Pediat, Seattle, WA 98195 USAUniv Washington, Dept Microbiol, Seattle, WA 98195 USA
机构:
Univ Washington, Dept Immunol, Seattle, WA 98195 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
Cambier, C. J.
;
Takaki, Kevin K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Microbiol, Seattle, WA 98195 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
Takaki, Kevin K.
;
Larson, Ryan P.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Immunol, Seattle, WA 98195 USA
Seattle Biomed Res Inst, Seattle, WA 98109 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
Larson, Ryan P.
;
Hernandez, Rafael E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Pediat, Seattle, WA 98195 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
Hernandez, Rafael E.
;
Tobin, David M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Microbiol, Seattle, WA 98195 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
Tobin, David M.
;
Urdahl, Kevin B.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Immunol, Seattle, WA 98195 USA
Seattle Biomed Res Inst, Seattle, WA 98109 USA
Univ Washington, Dept Pediat, Seattle, WA 98195 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
Urdahl, Kevin B.
;
Cosma, Christine L.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Microbiol, Seattle, WA 98195 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
Cosma, Christine L.
;
Ramakrishnan, Lalita
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Immunol, Seattle, WA 98195 USA
Univ Washington, Dept Microbiol, Seattle, WA 98195 USA
Univ Washington, Dept Med, Seattle, WA 98195 USAUniv Washington, Dept Immunol, Seattle, WA 98195 USA
机构:
Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
Broad Inst MIT & Harvard, Cambridge, MA 02142 USAMassachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Clatworthy, Anne E.
;
Lee, Jenny See-Wai
论文数: 0引用数: 0
h-index: 0
机构:
Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
Broad Inst MIT & Harvard, Cambridge, MA 02142 USAMassachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Lee, Jenny See-Wai
;
Leibman, Mark
论文数: 0引用数: 0
h-index: 0
机构:
Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
Broad Inst MIT & Harvard, Cambridge, MA 02142 USAMassachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Leibman, Mark
;
Kostun, Zachary
论文数: 0引用数: 0
h-index: 0
机构:
Massachusetts Gen Hosp, Ctr Regenerat Med, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Med, Boston, MA 02114 USAMassachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Kostun, Zachary
;
Davidson, Alan J.
论文数: 0引用数: 0
h-index: 0
机构:
Massachusetts Gen Hosp, Ctr Regenerat Med, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Med, Boston, MA 02114 USAMassachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Davidson, Alan J.
;
Hung, Deborah T.
论文数: 0引用数: 0
h-index: 0
机构:
Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Boston, MA 02114 USA
Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
Broad Inst MIT & Harvard, Cambridge, MA 02142 USA
Harvard Univ, Sch Med, Dept Med, Boston, MA 02114 USAMassachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
机构:
Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Univ Wisconsin, Dept Pediat, Madison, WI 53706 USAUniv Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Deng, Qing
;
Sarris, Milka
论文数: 0引用数: 0
h-index: 0
机构:
Inst Pasteur, Macrophages & Dev Immun Unit, Dept Dev & Stem Cell Biol, Paris, France
CNRS, URA2578, Paris, FranceUniv Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Sarris, Milka
;
Bennin, David A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Univ Wisconsin, Dept Pediat, Madison, WI 53706 USAUniv Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Bennin, David A.
;
Green, Julie M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Univ Wisconsin, Dept Pediat, Madison, WI 53706 USAUniv Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Green, Julie M.
;
Herbomel, Philippe
论文数: 0引用数: 0
h-index: 0
机构:
Inst Pasteur, Macrophages & Dev Immun Unit, Dept Dev & Stem Cell Biol, Paris, France
CNRS, URA2578, Paris, FranceUniv Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Herbomel, Philippe
;
Huttenlocher, Anna
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
Univ Wisconsin, Dept Pediat, Madison, WI 53706 USAUniv Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA