Venthamarai chooranam, a polyherbal Siddha medicine, alleviates hypertension via AT1R and eNOS signaling pathway in 2K1C hypertensive rats

被引：5
作者：
Babu, Chidambaram S. [1 ]
Kalaivani, Periyathambi [1 ]
Ranju, Vijayan [1 ]
Sathiya, Sekar [1 ]
Anbarasi, Chandrasekar [2 ]
Mahadevan, Murugappapillai V. [1 ]
Vijayakumar, Harivanzan [2 ]
Sunil, Adoor G. [1 ]
Thanikachalam, Sadagopan [1 ,2 ]
机构：
[1] Sri Ramachandra Univ, Ctr Toxicol & Dev Res CEFT, Chennai 600116, Tamil Nadu, India
[2] Sri Ramachandra Univ, PURSE HIS Study, Chennai 600116, Tamil Nadu, India
来源：
EXPERIMENTAL BIOLOGY AND MEDICINE | 2014年 / 239卷 / 06期
关键词：
Renovascular hypertension; Siddha system; 2K1C; Venthamarai chooranam; AT(1)R; AT(2)R; eNOS; NITRIC-OXIDE SYNTHASE; BLACK TEA POLYPHENOLS; OXIDATIVE STRESS; II TYPE-1; DEPENDENT PATHWAY; ENDOTHELIAL-CELLS; VASCULAR-DISEASE; NADPH OXIDASE; RECEPTOR; EXPRESSION;
D O I：
10.1177/1535370214525317
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The present study was aimed to scientifically demonstrate the anti-hypertensive action of Venthamarai chooranam (VMC) in renal hypertensive rats. Two Kidney One Clip (2K1C) Goldblatt model was adopted to induce hypertension in rats. Male Sprague Dawley rats (270-320 g) were randomized into sham (n = 6), vehicle-treated 2K1C (n = 9) and VMC-treated 2K1C (400 mg/kg, p.o; n = 8) and monitored for nine weeks. Systolic blood pressure (SBP), plasma nitrate/nitrite, carotid endothelial nitric oxide synthetase (eNOS), renal angiotensin type 1 receptor (AT(1)R), angiotensin type 2 receptor (AT(2)R), TNF alpha, IL-6, thioredoxin 1 (TRX1), and thioredoxin reductase 1 (TRXR1) mRNA expressions were studied. VMC upregulated eNOS expression which in turn improved plasma nitric oxide and decreased SBP in hypertensive rats. It down-regulated AT(1)R and simultaneously upregulated AT(2)R expression in comparison to vehicle-treated 2K1C rats. Further, renal TNF alpha and IL-6 expressions were down-regulated while TRX1 and TRXR1 were upregulated by VMC. VMC potentially interacts with renin-angiotensin components and endothelial functions, and thereby exerts its antihypertensive action. This is the first study to demonstrate the mechanism of anti-hypertensive action of VMC in an animal model of renovascular hypertension.
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收藏
页码：758 / 769
页数：12
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