Venthamarai chooranam, a polyherbal Siddha medicine, alleviates hypertension via AT1R and eNOS signaling pathway in 2K1C hypertensive rats

被引：5

作者：

Babu, Chidambaram S. ^{[1
]}

Kalaivani, Periyathambi ^{[1
]}

Ranju, Vijayan ^{[1
]}

Sathiya, Sekar ^{[1
]}

Anbarasi, Chandrasekar ^{[2
]}

Mahadevan, Murugappapillai V. ^{[1
]}

Vijayakumar, Harivanzan ^{[2
]}

Sunil, Adoor G. ^{[1
]}

Thanikachalam, Sadagopan ^{[1
,2
]}

机构：

[1] Sri Ramachandra Univ, Ctr Toxicol & Dev Res CEFT, Chennai 600116, Tamil Nadu, India

[2] Sri Ramachandra Univ, PURSE HIS Study, Chennai 600116, Tamil Nadu, India

来源：

EXPERIMENTAL BIOLOGY AND MEDICINE | 2014年 / 239卷 / 06期

关键词：

Renovascular hypertension; Siddha system; 2K1C; Venthamarai chooranam; AT(1)R; AT(2)R; eNOS; NITRIC-OXIDE SYNTHASE; BLACK TEA POLYPHENOLS; OXIDATIVE STRESS; II TYPE-1; DEPENDENT PATHWAY; ENDOTHELIAL-CELLS; VASCULAR-DISEASE; NADPH OXIDASE; RECEPTOR; EXPRESSION;

D O I：

10.1177/1535370214525317

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The present study was aimed to scientifically demonstrate the anti-hypertensive action of Venthamarai chooranam (VMC) in renal hypertensive rats. Two Kidney One Clip (2K1C) Goldblatt model was adopted to induce hypertension in rats. Male Sprague Dawley rats (270-320 g) were randomized into sham (n = 6), vehicle-treated 2K1C (n = 9) and VMC-treated 2K1C (400 mg/kg, p.o; n = 8) and monitored for nine weeks. Systolic blood pressure (SBP), plasma nitrate/nitrite, carotid endothelial nitric oxide synthetase (eNOS), renal angiotensin type 1 receptor (AT(1)R), angiotensin type 2 receptor (AT(2)R), TNF alpha, IL-6, thioredoxin 1 (TRX1), and thioredoxin reductase 1 (TRXR1) mRNA expressions were studied. VMC upregulated eNOS expression which in turn improved plasma nitric oxide and decreased SBP in hypertensive rats. It down-regulated AT(1)R and simultaneously upregulated AT(2)R expression in comparison to vehicle-treated 2K1C rats. Further, renal TNF alpha and IL-6 expressions were down-regulated while TRX1 and TRXR1 were upregulated by VMC. VMC potentially interacts with renin-angiotensin components and endothelial functions, and thereby exerts its antihypertensive action. This is the first study to demonstrate the mechanism of anti-hypertensive action of VMC in an animal model of renovascular hypertension.

引用

页码：758 / 769

页数：12

共 38 条

[1] Angiotensin AT2 receptors directly stimulate renal nitric oxide in bradykinin B2-receptor-null mice [J].