Expression and functional characterization of the putative protein 8b of the severe acute respiratory syndrome-associated coronavirus

被引：21

作者：

Law, Pui Ying Peggy

Liu, Yuet-Man

Geng, Hua

Kwan, Ka Ho

Waye, Mary Miu-Yee

Ho, Yuan-Yuan ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Dept Biochem, Shatin, Hong Kong, Peoples R China

[2] Chinese Univ Hong Kong, Mol Biotechnol Program, Shatin, Hong Kong, Peoples R China

[3] Chinese Univ Hong Kong, Croucher Lab Human Genom, Shatin, Hong Kong, Peoples R China

来源：

FEBS LETTERS | 2006年 / 580卷 / 15期

关键词：

severe acute respiratory syndrome; coronavirus; SARS ORF 8; SARS; 8b; X5;

D O I：

10.1016/j.febslet.2006.05.051

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SARS 8b is one of the putative accessory proteins of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) with unknown functions. In this study, the cellular localization and activity of this estimated 9.6 kDa protein were examined. Confocal microscopy results indicated that SARS 8b is localized in both nucleus and cytoplasm of mammalian cells. Functional study revealed that overexpression of SARS 8b induced DNA synthesis. Coexpression of SARS 8b and SARS 6, a previously characterized SARS-CoV accessory protein, did not elicit synergistic effects on DNA synthesis. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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页码：3643 / 3648

页数：6

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