A BOD biosensor based on a microorganism immobilized on an Al2O3s sol-gel matrix

被引:33
作者
Chen, DD [1 ]
Cao, YB [1 ]
Liu, BH [1 ]
Kong, JL [1 ]
机构
[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
基金
中国国家自然科学基金;
关键词
BOD biosensors; microorganism; sol-gel;
D O I
10.1007/s00216-001-1214-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A novel BOD biosensor has been fabricated from a microbial membrane on an oxygen electrode; a porous inorganic Al2O3 sol-gel matrix was used to immobilize the yeast. Use of the sensor is convenient and rapid compared with the official BOD5 method. The gelation time required to form the microorganism membrane was less than 15 min. There was linear relationship between the response (sensor current) and BOD values ranging from 10-50 mg L-1. The lifetime of the BOD biosensor was more than 30 days at ambient temperature.
引用
收藏
页码:737 / 739
页数:3
相关论文
共 10 条
  • [1] ALEX WK, 1998, ANAL LETT, V31, P2309
  • [2] *AM PUBL HLTH ASS, 1989, STAND METH
  • [3] Development of highly sensitive BOD sensor and its evaluation using preozonation
    Chee, GJ
    Nomura, Y
    Ikebukuro, K
    Karube, I
    [J]. ANALYTICA CHIMICA ACTA, 1999, 394 (01) : 65 - 71
  • [4] Biosensor for the estimation of low biochemical oxygen demand
    Chee, GJ
    Nomura, Y
    Karube, I
    [J]. ANALYTICA CHIMICA ACTA, 1999, 379 (1-2) : 185 - 191
  • [5] SOL-GEL ENCAPSULATION METHODS FOR BIOSENSORS
    DAVE, BC
    DUNN, B
    VALENTINE, JS
    ZINK, JI
    [J]. ANALYTICAL CHEMISTRY, 1994, 66 (22) : A1120 - A1127
  • [6] *JAP IND STAND COM, 1989, K0102 JIS COMM
  • [7] LEV O, 1995, ANAL CHEM, V67, P22
  • [8] A new tyrosinase biosensor based on tailoring the porosity of A12O3 sol-gel to co-immobilize tyrosinase and the mediator
    Liu, ZJ
    Deng, JQ
    Li, D
    [J]. ANALYTICA CHIMICA ACTA, 2000, 407 (1-2) : 87 - 96
  • [9] Al2O3 sol-gel derived amperometric biosensor for glucose
    Liu, ZJ
    Liu, BH
    Zhang, M
    Kong, JL
    Deng, JQ
    [J]. ANALYTICA CHIMICA ACTA, 1999, 392 (2-3) : 135 - 141
  • [10] Measurement of biodegradable substances with a mycelia-sensor based on the salt tolerant yeast Arxula adeninivorans LS3
    Tag, K
    Lehmann, M
    Chan, CY
    Renneberg, R
    Riedel, K
    Kunze, G
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2000, 67 (1-2) : 142 - 148