CDK14 Contributes to Reactive Gliosis via Interaction with Cyclin Y in Rat Model of Spinal Cord Injury

被引:12
作者
Duan, Chengwei [1 ]
Liu, Yonghua [2 ]
Lu, Lu [3 ]
Cai, Rixin [3 ]
Xue, Huaqing [1 ]
Mao, Xingxing [4 ]
Chen, Chen [4 ]
Qian, Rong [4 ]
Zhang, Dongmei [2 ]
Shen, Aiguo [1 ]
机构
[1] Nantong Univ, Jiangsu Prov Key Lab Neuroregenerat, Nantong 226001, Jiangsu, Peoples R China
[2] Nantong Univ, Dept Pathogen Biol, Coll Med, Jiangsu Prov Key Lab Inflammat & Mol Drug Target, Nantong 226001, Jiangsu, Peoples R China
[3] Nantong Univ, Dept Histol & Embryol, Coll Med, Nantong 226001, Jiangsu, Peoples R China
[4] Nantong Univ, Dept Orthopaed, Affiliated Hosp, Nantong 226001, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
CDK14; Cyclin Y; Spinal cord injury; TRAUMATIC BRAIN-INJURY; CELL-CYCLE; CONSERVED CYCLIN; NERVOUS-SYSTEM; SCAR FORMATION; REGENERATION; ASTROCYTES; PROTEINS; SUPPRESSION; ACTIVATION;
D O I
10.1007/s12031-015-0639-x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cyclin-dependent kinases (CDKs) are perceived as the engine that drives cell cycle progression whereas cyclins are considered to be the gears that are changed to aid the transition between cycle phases. CDK14 is a cdc2-related serine/threonine protein kinase and plays an important role in normal cell cycle progression. However, its distribution and function in the central nervous system (CNS) lesion remain unclear. In this study, we mainly investigated the protein expression and cellular localization of CDK14 during spinal cord injury (SCI). Western blot analysis revealed that the expression of CDK14 was gradually increased and reached a peak at 3 days after SCI. The expression of CDK14 was further analyzed by immunohistochemistry. Double immunofluorescence staining showed that CDK14 was co-expressed prominent in astrocytes. Co-localization CDK14/proliferating cell nuclear antigen (PCNA) were detected in glial cells. cyclin Y, which can interact with CDK14, was detected that had same expression trend was consistent with CDK14 Western blot results in SCI. Double-immunofluorescence staining indicated that CDK14 co-expressed with cyclin Y in some cells. Co-immunoprecipitation had been showed that CDK14 could interact with cyclin Y after acute SCI. Taken together, these data suggested that both CDK14 and cyclin Y may play important roles in spinal cord pathophysiology.
引用
收藏
页码:571 / 579
页数:9
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