Identification of Odor Impact Compounds of Tagetes minuta L. Essential Oil: Comparison of Two GC-Olfactometry Methods

Odor impact compounds of Tagetes minuta L. essential Oil Were studied by gas chromatography (GC)-olfactometry using aroma extract dilution analysis (AEDA) and vocabulary-intensity-duration of elementary odors by sniffing (VIDEO-Sniff). AEDA was conducted by direct injection and revealed the presence of odorant ones Highest flavor dilution (FD) values were obtained for ethyl 2-met hyl ro anoate ethyl methyl utanoate (E)-ocimenone, two tentatively identified thiols, and two yet unknown compounds VIDEO Sniff as realized by dynamic ea s ace sampling (D-HS) combined with 8W-GC-olfactometry where eight sniffers simultaneously detect volatile compounds obtained from a single chromatographic separation and revealed the presence of odorant ones Odorant trace compounds detected by GC O that were present in quantities inferior to the GC MS systems detection limit and those subject to coelutions were identified by GCxGC time of flight mass spectrometry (TOFMS). A total amount of 37 odorant components could be identified by VIDEO Sniff an the strong influence of the fruity notes of numerous esters stood out Highest olfactory signals were obtained for ethyl methyl roanoate etyl and methyl utanoate and oct en one Bot met o s ence come to the conclusion to at ethyl methyl r anciate and ethyl and methyl utanoate are among the main odorants in Tagetes minuta L. essential oil. Differences advantages and ra ac s of ot GC 0 met o s are discussed.