Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae

被引：28
|
作者：
Qi, Xin [1 ,2 ]
Zha, Jian [1 ,2 ]
Liu, Gao-Gang [1 ,2 ]
Zhang, Weiwen [1 ,2 ]
Li, Bing-Zhi [1 ,2 ]
Yuan, Ying-Jin [1 ,2 ]
机构：
[1] Tianjin Univ, Minist Educ, Key Lab Syst Bioengn, Tianjin 300072, Peoples R China
[2] Tianjin Univ, Sch Chem Engn & Technol, Collaborat Innovat Ctr Chem Sci & Engn Tianjin, SynBio Res Platform, Tianjin 300072, Peoples R China
来源：
FRONTIERS IN MICROBIOLOGY | 2015年 / 6卷
关键词：
synthetic biology; xylose isomerase; XylA; xylose utilization; yeast; evolutionary engineering; PENTOSE-PHOSPHATE PATHWAY; INCREASES ETHANOL-PRODUCTION; FUNCTIONAL EXPRESSION; XYLULOKINASE ACTIVITY; ENHANCED EXPRESSION; ADAPTIVE EVOLUTION; ESCHERICHIA-COLI; METABOLIC FLUX; CORN STOVER; FERMENTATION;
D O I：
10.3389/fmicb.2015.01165
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is a promising approach to engineering heterologous pathway for xylose utilization in Saccharomyces cerevisiae. Here, we constructed a xylose-fermenting yeast SyBE001 through combinatorial fine-tuning the expression of XylA and endogenous XKS1. Additional overexpression of genes RK11, RPE1, TKL1, and TAL1 in the non-oxidative pentose phosphate pathway (PPP) in SyBE001 increased the xylose consumption rate by 1.19-fold. By repetitive adaptation, the xylose utilization rate was further increased by similar to 10-fold in the resultant strain SyBE003. Gene expression analysis identified a variety of genes with significantly changed expression in the PPP, glycolysis and the tricarboxylic acid cycle in SyBE003.
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页数：12
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