An Artificial Imine Reductase based on the Ribonuclease S Scaffold

被引：19

作者：

Genz, Maika ^{[1
]}

Koehler, Valentin ^{[2
]}

Krauss, Michel ^{[1
]}

Singer, David ^{[1
]}

Hoffmann, Ralf ^{[1
]}

Ward, Thomas R. ^{[2
]}

Straeter, Norbert ^{[1
]}

机构：

[1] Univ Leipzig, Ctr Biotechnol & Biomed, Inst Bioanalyt Chem, D-04103 Leipzig, Germany

[2] Univ Basel, Dept Chem, CH-4056 Basel, Switzerland

来源：

CHEMCATCHEM | 2014年 / 6卷 / 03期

关键词：

asymmetric catalysis; half-sandwich complexes; metalloenzymes; reduction; transfer hydrogenation; ASYMMETRIC TRANSFER HYDROGENATION; CYCLIC IMINES; BIOCATALYTIC ROUTES; CRYSTAL-STRUCTURE; PROTEIN; METALLOENZYMES; EVOLUTION; CATALYSTS; DESIGN; RESOLUTION;

D O I：

10.1002/cctc.201300995

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Dative anchoring of a piano-stool complex within ribonucleaseS resulted in an artificial imine reductase. The catalytic performance was modulated upon variation of the coordinating amino acid residues in the S-peptide. Binding of Cp*Ir (Cp*=C5Me5) to the native active site resulted in good conversions and moderate enantiomeric excess values for the synthesis of salsolidine.

引用

页码：736 / 740

页数：5

共 40 条

[1]

[Anonymous], 2011, ANGEW CHEM INT ED, V50, P3026

[2]

[Anonymous], 2009, ANGEW CHEM, DOI DOI 10.1002/ANGE.200902570

[3]

[Anonymous], 2009, ANGEW CHEM INT EDIT, V48, P6524

[4]

[Anonymous], 2012, ANGEW CHEM INT ED, V51, P7472

[5] Engineering the third wave of biocatalysis [J].

Bornscheuer, U. T. ;

Huisman, G. W. ;

Kazlauskas, R. J. ;

Lutz, S. ;

Moore, J. C. ;

Robins, K. .

NATURE, 2012, 485 (7397) :185-194

[6]

Coquiere D., 2009, ANGEW CHEM, V121, P5261

[7] Designed evolution of artificial metalloenzymes: protein catalysts made to order [J].

Creus, Marc ;

Ward, Thomas R. .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2007, 5 (12) :1835-1844

[8]

Durrenberger M., 2011, ANGEW CHEMIE, V123, P3082

[9] A Highly Enantioselective Access to Tetrahydroisoquinoline and β-Carboline Alkaloids with Simple Noyori-Type Catalysts in Aqueous Media [J].

Evanno, Laurent ;

Ormala, Joel ;

Pihko, Petri M. .

CHEMISTRY-A EUROPEAN JOURNAL, 2009, 15 (47) :12963-12967

[10] Crystal Structure of Apo- and Metalated Thiolate containing RNase S as Structural Basis for the Design of Artificial Metalloenzymes by Peptide-Protein Complementation [J].

Genz, Maika ;

Singer, David ;

Hey-Hawkins, Evamarie ;

Hoffmann, Ralf ;

Straeter, Norbert .

ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE, 2013, 639 (14) :2395-2400

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