A simple method for human peripheral blood monocyte isolation

被引：92

作者：

de Almeida, MC

Silva, AC

Barral, A

Netto, MB

机构：

[1] FIOCRUZ, Ctr Pesquisas Goncalo Moniz, BR-40295001 Salvador, BA, Brazil

[2] Univ Brasilia, FCS, Dept Patol, BR-70910900 Brasilia, DF, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2000年 / 95卷 / 02期

关键词：

monocytes; percoll; gradient-isolation;

D O I：

10.1590/S0074-02762000000200014

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy, coat cells. The use of simple laboratory equipment and a relatively cheap I reagent makes the described method a convenient approach to obtaining human monocytes.

引用

页码：221 / 223

页数：3

共 12 条

[1]

BENNETT S, 1994, J LEUKOCYTE BIOL, V56, P236

[2] MONOCYTE PROLIFERATION IN A CYTOKINE-FREE, SERUM-FREE SYSTEM [J].