In vitro and in vivo evaluation of bone formation using solid freeform fabrication-based bone morphogenic protein-2 releasing PCL/PLGA scaffolds

被引：33

作者：

Kim, Tae-Hoon ^{[1
]}

Yun, Young-Pil ^{[2
,3
]}

Park, Young-Eun ^{[2
,3
]}

Lee, Suk-Ha ^{[1
]}

Yong, Woonjae ^{[4
]}

Kundu, Joydip ^{[4
]}

Jung, Jin Woo ^{[4
]}

Shim, Jin-Hyung ^{[4
]}

Cho, Dong-Woo ^{[4
,5
]}

Kim, Sung Eun ^{[2
,3
]}

Song, Hae-Ryong ^{[2
,3
]}

机构：

[1] Konkuk Univ, Sch Med, Dept Orthoped Surg, Seoul 143729, South Korea

[2] Korea Univ, Coll Med, Guro Hosp, Dept Orthoped Surg, Seoul 152703, South Korea

[3] Korea Univ, Coll Med, Guro Hosp, Rare Dis Inst, Seoul 152703, South Korea

[4] Pohang Univ Sci & Technol POSTECH, Dept Mech Engn, Pohang 790784, Kyungbuk, South Korea

[5] Pohang Univ Sci & Technol POSTECH, Div Integrat Biosci & Biotechnol, Pohang 790784, South Korea

来源：

BIOMEDICAL MATERIALS | 2014年 / 9卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

bone morphogenic protein-2 (BMP-2); solid; freeform fabrication (SFF); osteoblast activity; bone formation; polycaprolactone (PCL); poly(lactic-co-glycolic acid) (PLGA); ANGIOGENIC GROWTH-FACTOR; LUMBAR INTERBODY FUSION; MESENCHYMAL STEM-CELLS; MARROW STROMAL CELLS; POROUS SCAFFOLDS; OSTEOBLAST FUNCTIONS; CALCIUM PHOSPHATES; BACTERIAL ADHESION; PLGA MICROSPHERES; FACTOR DELIVERY;

D O I：

10.1088/1748-6041/9/2/025008

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The aim of this study was to develop novel polycaprolactone/poly(lactic-co-glycolic acid) (PCL/PLGA) scaffolds with a heparin-dopamine (Hep-DOPA) conjugate for controlled release of bone morphogenic protein-2 (BMP-2) to enhance osteoblast activity in vitro and also bone formation in vivo. PCL/PLGA scaffolds were prepared by a solid freeform fabrication method. The PCL/PLGA scaffolds were functionalized with Hep-DOPA and then BMP-2 was sequentially coated onto the Hep-DOPA/PCL/PLGA scaffolds. The characterization and surface elemental composition of all scaffolds were evaluated by scanning electron microscope and x-ray photoelectron spectroscopy. The osteoblast activities on all scaffolds were assessed by cell proliferation, alkaline phosphatase (ALP) activity and calcium deposition in vitro. To demonstrate bone formation in vivo, plain radiograph, micro-computed tomography (micro-CT) evaluation and histological studies were performed after the implantation of all scaffolds on a rat femur defect. Hep-DOPA/PCL/PLGA had more controlled release of BMP-2, which was quantified by enzyme-linked immunosorbent assay, compared with Hep/PCL/PLGA. The in vitro results showed that osteoblast-like cells (MG-63 cells) grown on BMP-2/Hep-DOPA/PCL/PLGA had significantly enhanced ALP activity and calcium deposition compared with those on BMP-2/Hep/PCL/PLGA and PCL/PLGA. In addition, the plain radiograph, micro-CT evaluation and histological studies demonstrated that the implanted BMP-2/Hep-DOPA/PCL/PLGA on rat femur had more bone formation than BMP-2/Hep/PCL/PLGA and PCL/PLGA in vivo.

引用

页数：11

共 49 条

[1] Osteogenic differentiation of dura mater stem cells cultured in vitro on three-dimensional porous scaffolds of poly(ε-caprolactone) fabricated via co-extrusion and gas foaming [J].