Analysis of Cytosolic pH Changes in Thymocytes During Early Apoptosis with Improved Three-Channel Real-Time Fluorescence Imaging

被引：3

作者：

Zhang Shu ^{[1
]}

Liu Xiaochen ^{[1
]}

Deng Chuyun ^{[1
,3
]}

Han Man ^{[2
]}

Pan Juhua ^{[2
]}

Liao Xinghua ^{[2
]}

Qi Xin ^{[2
]}

Duan Shaojin ^{[2
]}

Ma Wanyun ^{[1
,4
]}

机构：

[1] Tsinghua Univ, Dept Phys, State Key Lab Low Dimens Quantum Phys, Beijing 100084, Peoples R China

[2] Guang An Men Hosp, Beijing 100053, Peoples R China

[3] Natl Univ Def Technol, Sch Sci, Changsha 410073, Hunan, Peoples R China

[4] Collaborat Innovat Ctr Quantum Matter, Beijing, Peoples R China

来源：

JOURNAL OF FLUORESCENCE | 2014年 / 24卷 / 04期

关键词：

Three-channel; Spectral crosstalk; Real-time fluorescence imaging; Apoptosis; Cytosolic pH; INTRACELLULAR PH; MICROSCOPY; CELLS; GSNO; CARBOXY-SNARF-1;

D O I：

10.1007/s10895-014-1384-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytosolic pH changes in single thymocytes in the early stages of apoptosis were examined with spectral-crosstalk-improved three-channel real-time fluorescence imaging. Spectral crosstalk was greatly reduced by this improved image processing method. Compared with normal thymocytes, the improved image processing method showed that the cytosolic pH value of thymocytes undergoing apoptosis was lower. A slight diversity in the intracellular pH values of samples subjected to different drug treatments was also observed. This new image processing method is beneficial to the application of the three-channel real-time fluorescence imaging to biological processes.

引用

页码：1055 / 1059

页数：5

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