Isolation and identification of novel propoxyphenyl thiosildenafil found in natural health food product

被引：20

作者：

Han, Kyoung Moon ^{[1
]}

Lee, Ji Hyun ^{[1
]}

Park, Hyoung-Joon ^{[1
]}

Hwang, Insun ^{[1
]}

Heo, Ok-Soon ^{[1
]}

Kim, Woo Seong ^{[1
]}

机构：

[1] Minist Food & Drug Safety, Natl Inst Food & Drug Safety Evaluat, Toxicol Evaluat & Res Dept, Adv Anal Team, Cheongwon Gun, South Korea

来源：

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT | 2014年 / 31卷 / 01期

关键词：

thiosildenafil analogue; propoxyphenyl thiosildenafil; LC-TOF; MS; NMR; HERBAL DIETARY-SUPPLEMENTS; STRUCTURE ELUCIDATION; SILDENAFIL; ANALOG; VARDENAFIL; TADALAFIL; ADULTERANT;

D O I：

10.1080/19440049.2013.855948

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A novel phosphodiesterase-5 (PDE-5) inhibitor was found in a natural health food product. The previously unknown sildenafil analogue was isolated using preparative HPLC. The structure of the compound was elucidated using HPLC with diode array detection (DAD), time-of-flight mass spectrometry (TOF/MS), and nuclear magnetic resonance spectroscopy (NMR). An [M+H](+) ion was detected at m/z 505.2077 by LC-TOF/MS that was consistent with C23H32N6O3S2 (-0.98ppm). By NMR analysis, the analogue was identified as 1-methyl-5-(5-(4-methylpiperazin-1-ylsulfonyl)-2-propoxyphenyl)-3-propyl-1H-pyrazolo[4,3-d]pyrimidine-7(6H)-thione. In this structure, the ethoxy group of thiosildenafil was substituted by a propoxy group of the unknown compound. Therefore, this novel thiosildenafil analogue was named propoxyphenyl thiosildenafil.

引用

页码：1 / 6

页数：6

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