On the fundamental imaging-depth limit in two-photon microscopy

被引：314

作者：

Theer, Patrick ^{[1
]}

Denk, Winfried ^{[1
]}

机构：

[1] Max Planck Inst Med Res, D-69120 Heidelberg, Germany

来源：

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION | 2006年 / 23卷 / 12期

关键词：

D O I：

10.1364/JOSAA.23.003139

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have analyzed how the maximal imaging depth of two-photon microscopy in scattering samples depends on properties of the sample and the imaging system. We find that the imaging depth increases with increasing numerical aperture and staining inhomogeneity and with decreasing excitation-pulse duration and scattering anisotropy factor, but is ultimately limited by near-surface fluorescence with slight improvements possible using special detection strategies. (c) 2006 Optical Society of America.

引用

页码：3139 / 3149

页数：11

共 50 条

[1] On the fundamental imaging-depth limit in two-photon microscopy
Theer, P
Denk, W
FEMTOSECOND LASER APPLICATIONS IN BIOLOGY, 2004, 5463 : 45 - 55
[2] Comparing the fundamental imaging depth limit of two-photon, three-photon, and non-degenerate two-photon microscopy
Cheng, Xiaojun
Sadegh, Sanaz
Zilpelwar, Sharvari
Devor, Anna
Tian, Lei
Boas, David A.
OPTICS LETTERS, 2020, 45 (10) : 2934 - 2937
[3] Extending the fundamental imaging-depth limit of multi-photon microscopy by imaging with photo-activatable fluorophores
Chen, Zhixing
Wei, Lu
Zhu, Xinxin
Min, Wei
OPTICS EXPRESS, 2012, 20 (17): : 18525 - 18536
[4] Scattering effect on the imaging depth limit in two-photon fluorescence microscopy
Sergeeva, E. A.
QUANTUM ELECTRONICS, 2010, 40 (05) : 411 - 417
[5] Stimulated emission reduced fluorescence microscopy: a concept for extending the fundamental depth limit of two-photon fluorescence imaging
Wei, Lu
Chen, Zhixing
Min, Wei
BIOMEDICAL OPTICS EXPRESS, 2012, 3 (06): : 1465 - 1475
[6] Fundamental of two-photon microscopy
Nakamura, O
MICROSCOPY RESEARCH AND TECHNIQUE, 1999, 47 (03) : 165 - 171
[7] Superficial Bound of the Depth Limit of Two-Photon Imaging in Mouse Brain
Takasaki, Kevin
Abbasi-Asl, Reza
Waters, Jack
ENEURO, 2020, 7 (01)
[8] Two-photon microscopy in brain tissue: parameters influencing the imaging depth
Oheim, M
Beaurepaire, E
Chaigneau, E
Mertz, J
Charpak, S
JOURNAL OF NEUROSCIENCE METHODS, 2001, 111 (01) : 29 - 37
[9] Extended depth of field microscopy for rapid volumetric two-photon imaging
Theriault, Gabrielle
De Koninck, Yves
McCarthy, Nathalie
OPTICS EXPRESS, 2013, 21 (08): : 10095 - 10104
[10] Maximum imaging depth of two-photon autofluorescence microscopy in epithelial tissues
Durr, Nicholas J.
Weisspfennig, Christian T.
Holfeld, Benjamin A.
Ben-Yakar, Adela
JOURNAL OF BIOMEDICAL OPTICS, 2011, 16 (02)

← 1 2 3 4 5 →