GDNF secreted by pre-osteoclasts induces migration of bone marrow mesenchymal stem cells and stimulates osteogenesis

被引：12
|
作者：
Yi, Sol [1 ,2 ]
Kim, Jihee [1 ,2 ]
Lee, Soo Young [1 ,2 ]
机构：
[1] Ewha Womans Univ, Dept Life Sci, Seoul 03760, South Korea
[2] Ewha Womans Univ, Res Ctr Cellular Homeostasis, Seoul 03760, South Korea
来源：
BMB REPORTS | 2020年 / 53卷 / 12期
基金：
新加坡国家研究基金会;
关键词：
Bone homeostasis; Bone marrow mesenchymal stem cell; Cell migration; Glial cell-derived neurotrophic factor; Osteogenesis; NEUROTROPHIC FACTOR; RECEPTOR ACTIVATOR; PREOSTEOCLASTS; ANGIOGENESIS; RESORPTION; FAMILY;
D O I：
10.5483/BMBRep.2020.53.12.199
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bone resorption is linked to bone formation via temporal and spatial coupling within the remodeling cycle. Several lines of evidence point to the critical role of coupling factors derived from pre-osteoclasts (POCs) during the regulation of bone marrow-derived mesenchymal stem cells (BMMSCs). However, the role of glial cell-derived neurotrophic factor (GDNF) in BMMSCs is not completely understood. Herein, we demonstrate the role of POC-derived GDNF in regulating the migration and osteogenic differentiation of BMMSCs. RNA sequencing revealed GDNF upregulation in POCs compared with monocytes/macrophages. Specifically, BMMSC migration was inhibited by a neutralizing antibody against GDNF in pre-osteoclast-conditioned medium (POC-CM), whereas treatment with a recombinant GDNF enhanced migration and osteogenic differentiation. In addition, POC-CM derived from GDNF knock-downed bone marrow macrophages suppressed BMMSC migration and osteogenic differentiation. SPP86, a small molecule inhibitor, inhibits BMMSC migration and osteogenic differentiation by targeting the receptor tyrosine kinase RET, which is recruited by GDNF into the GFR alpha 1 complex. Overall, this study highlights the role of POC-derived GDNF in BMMSC migration and osteogenic differentiation, suggesting that GDNF regulates bone metabolism.
引用
收藏
页码：646 / 651
页数：6
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