Streptomycetes cultured on glass beads:: Sample preparation for SEM

被引：11

作者：

Kofronová, O ^{[1
]}

Nguyen, LD ^{[1
]}

Weiser, J ^{[1
]}

Benada, O ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Microbiol, CR-14220 Prague, Czech Republic

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2002年 / 58卷 / 02期

关键词：

scanning electron microscopy; streptomycetes; glass beads; low melting agarose; sample preparation;

D O I：

10.1002/jemt.10126

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

We demonstrate the preparation of samples of streptomycetes (Streptomyces coelicolor, S. aureofaciens) cultured on glass beads (balotina) for scanning electron microscopy. The main trick of the method consists in immobilization of glass beads with low-melting agarose. The samples are then fixed in OsO4 vapors followed by dehydration in vapors of absolute ethanol. No air-to-liquid transition during the sample preparation occurs. Consequently, whole cell cycle of streptomycetes in the term of mycelial morphology can readily be studied by this method. (C) 2002 Wiley-Liss, Inc.

引用

页码：111 / 113

页数：3