Paper-based molecular diagnostic for Chlamydia trachomatis

被引：69

作者：

Linnes, Jacqueline C. ^{[1
]}

Fan, Andy ^{[1
]}

Rodriguez, Natalia M. ^{[1
]}

Lemieux, Bertrand ^{[2
]}

Kong, Huimin ^{[2
]}

Klapperich, Catherine M. ^{[1
]}

机构：

[1] Boston Univ, Boston, MA 02215 USA

[2] BioHelix Corp, Beverly, MA 01915 USA

来源：

RSC ADVANCES | 2014年 / 4卷 / 80期

关键词：

SEXUALLY-TRANSMITTED-DISEASES; NUCLEIC-ACIDS; AMPLIFICATION; POINT; DNA; TRANSMISSION; EXTRACTION; DEVICE;

D O I：

10.1039/c4ra07911f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein we show the development of a minimally instrumented paper-based molecular diagnostic for point of care detection of sexually transmitted infections caused by Chlamydia trachomatis. This new diagnostic platform incorporates cell lysis, isothermal nucleic acid amplification, and lateral flow visual detection using only pressure and heat sources, eliminating the need for expensive laboratory equipment. This paper-based test can be performed in less than one hour and has a clinically relevant limit of detection that is 100x more sensitive than current rapid immunoassays used for chlamydia diagnosis.

引用

收藏

页码：42245 / 42251

页数：7

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