Complete genome sequence of a new achyranthes virus A isolate fromAchyranthes bidentatain China

被引:2
作者
Wu, Xinyang [1 ,2 ]
Lai, Yuchao [2 ]
Lv, Lanqing [2 ]
Han, Kelei [2 ]
Chen, Ziqiang [2 ]
Lu, Yuwen [2 ]
Peng, Jiejun [2 ]
Lin, Lin [2 ]
Chen, Jianping [1 ,2 ]
Zheng, Hongying [2 ]
Yan, Fei [2 ]
机构
[1] Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou 310058, Peoples R China
[2] Ningbo Univ, State Key Lab Managing Biot & Chem Threats Qual &, Key Lab Biotechnol Plant Protect, Minist Agr & Zhejiang Prov,Inst Plant Virol, Ningbo 315211, Peoples R China
关键词
MOTTLE VIRUS; PCR PRIMER; POTYVIRUS;
D O I
10.1007/s00705-020-04839-5
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have determined the complete genomic sequence of a potyvirus fromAchyranthes bidentatain Zhejiang, China, using reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The genomic RNA is 9482 nucleotides (nt) long excluding the 3 '-terminal poly(A) tail and encodes a putative large polyprotein with 3073 amino acids (aa). It has 75.4-53.5% nt sequence identity and 84.0-49.1% polyprotein sequence identity to other potyviruses and is probably a distantly related isolate of the same species as the recently reported achyranthes virus A isolate from South Korea (AcVA-SK). This is the first report of the occurrence of a potyvirus infectingA. bidentatain China.
引用
收藏
页码:287 / 290
页数:4
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