Accurate measurement of long-range heteronuclear coupling constants from undistorted multiplets of an enhanced CPMG-HSQMBC experiment

被引：49

作者：

Kövér, KE

Batta, G

Fehér, K

机构：

[1] Univ Debrecen, Dept Inorgan & Analyt Chem, H-4010 Debrecen, Hungary

[2] Univ Debrecen, Hungarian Acad Sci, Dept Pharmaceut Chem, H-4010 Debrecen, Hungary

[3] Univ Debrecen, Hungarian Acad Sci, Res Grp Antibiot, H-4010 Debrecen, Hungary

[4] Max Planck Inst Med Res, Dept Biomol Mech, D-69120 Heidelberg, Germany

来源：

JOURNAL OF MAGNETIC RESONANCE | 2006年 / 181卷 / 01期

基金：

匈牙利科学研究基金会;

关键词：

long-range heteronuclear couplings; HSQMBC; CPMG; composite pulses; carbohydrate; glycopeptide;

D O I：

10.1016/j.jmr.2006.03.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we present a modified CPMG-HSQMBC experiment which is capable to reduce the detrimental phase twists in the "longrange" connectivity multiplets caused by proton-proton couplings. We demonstrate that concerted CPMG pulse trains applied on both nuclei in the starting CPMG-INEPT transfer step can considerably be improved by composite pi pulses that compensate for pulse imperfections and off-resonance effects. Experimental optimization of the interpulse delay within the CPMG cycle was found to be crucial in order to achieve the best possible "decoupling" of homonuclear coupling modulation. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：89 / 97

页数：9

共 11 条

[1] Low-power composite CPMG HSQMBC experiment for accurate measurement of long-range heteronuclear coupling constants
Boros, Sandor
Koever, Katalin E.
MAGNETIC RESONANCE IN CHEMISTRY, 2011, 49 (03) : 106 - 110
[2] PSYCHE CPMG-HSQMBC: An NMR Spectroscopic Method for Precise and Simple Measurement of Long-Range Heteronuclear Coupling Constants
Timari, Istvan
Szilagyi, Laszlo
Koever, Katalin E.
CHEMISTRY-A EUROPEAN JOURNAL, 2015, 21 (40) : 13939 - 13942
[3] Precise Measurement of Long-Range Heteronuclear Coupling Constants by a Novel Broadband Proton-Proton-Decoupled CPMG-HSQMBC Method
Timari, Istvan
Illyes, Tuende Z.
Adams, Ralph W.
Nilsson, Mathias
Szilagyi, Laszlo
Morris, Gareth A.
Koever, Katalin E.
CHEMISTRY-A EUROPEAN JOURNAL, 2015, 21 (08) : 3472 - 3479
[4] IPAP-HSQMBC: Measurement of long-range heteronuclear coupling constants from spin-state selective multiplets
Gil, Sergi
Felix Espinosa, Juan
Parella, Teodor
JOURNAL OF MAGNETIC RESONANCE, 2010, 207 (02) : 312 - 321
[5] Accurate measurement of small heteronuclear coupling constants from pure-phase α/β HSQMBC cross-peaks
Gil, Sergi
Felix Espinosa, Juan
Parella, Teodor
JOURNAL OF MAGNETIC RESONANCE, 2011, 213 (01) : 145 - 150
[6] Efficient measurement of the sign and the magnitude of long-range proton-carbon coupling constants from a spin-state-selective HSQMBC-COSY experiment
Sauri, Josep
Parella, Teodor
MAGNETIC RESONANCE IN CHEMISTRY, 2012, 50 (11) : 717 - 721
[7] Sensitivity enhancement of a two-dimensional experiment for the measurement of heteronuclear long-range coupling constants, by a new scheme of coherence selection by gradients
Cicero, DO
Barbato, G
Bazzo, R
JOURNAL OF MAGNETIC RESONANCE, 2001, 148 (01) : 209 - 213
[8] Measurement of long-range proton-carbon coupling constants from pure in-phase 1D multiplets
Espinosa, Juan F.
Vidal, Paloma
Parella, Teodor
Gil, Sergi
MAGNETIC RESONANCE IN CHEMISTRY, 2011, 49 (08) : 502 - 507
[9] Accurate measurement of long range proton-carbon scalar coupling constants
Dickson, Claire L.
Blundell, Charles D.
Butts, Craig P.
Felton, Alice
Jeffreys, Alex
Takacs, Zoltan
ANALYST, 2017, 142 (04) : 621 - 633
[10] Sensitivity- and gradient-enhanced hetero (ω1) half-filtered TOCSY experiment for measuring long-range coupling constants
Uhrín, D
Batta, G
Hruby, VJ
Barlow, PN
Kövér, KE
JOURNAL OF MAGNETIC RESONANCE, 1998, 130 (02) : 155 - 161

← 1 2 →