Development of PCR assays for the detection and differentiation of Fusarium sporotrichioides and Fusarium langsethiae

被引：85
作者：
Wilson, A
Simpson, D
Chandler, E
Jennings, P
Nicholson, P
机构：
[1] John Innes Inst, Dept Dis & Stress Biol, Norwich NR4 7UH, Norfolk, England
[2] John Innes Inst, Dept Metab Biol, Norwich NR4 7UH, Norfolk, England
[3] Cent Sci Lab, York YO41 1LZ, N Yorkshire, England
来源：
FEMS MICROBIOLOGY LETTERS | 2004年 / 233卷 / 01期
关键词：
Fusarium; PCR; trichothecenes;
D O I：
10.1016/j.femsle.2004.01.040
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Isolates of the type-A trichothecene producing Fusarium sporotrichioides and Fusarium langsethiae were grouped and differentiated in a phylogenetic tree using ITS sequence dissimilarity. An attempt was made to develop a PCR-based assay for the detection and differentiation of Fusarium sporotrichiodes from other Fusarium species using the 5'-region of the tri5 gene as a template. However, this assay was unable to differentiate, to a satisfactory level, between isolates of Fusarium sporotrichioides and Fusarium langsethiae, providing further genetic evidence for their close genetic relationship. A robust and repeatable PCR-assay was developed for the detection and differentiation of both species based on sequence determined from differentially amplified RAPD-PCR products. These assays were able to detect both species in samples of grain taken from the field. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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页码：69 / 76
页数：8
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