Advances in Raman imaging combined with AFM and fluorescence microscopy are beneficial for oncology and cancer research

被引：25
作者：
Abramczyk, Halina [1 ]
Imiela, Anna [1 ]
Brozek-Pluska, Beata [1 ]
Kopec, Monika [1 ]
机构：
[1] Lodz Univ Technol, Fac Chem, Inst Appl Radiat Chem, Lab Laser Mol Spect, Wroblewskiego 15, PL-93590 Lodz, Poland
来源：
NANOMEDICINE | 2019年 / 14卷 / 14期
关键词：
adhesion; AFM; brain tumor; breast cancer; Raman imaging; stiffness; BREAST-CANCER; SPECTROSCOPY; CELLS; MAMMAGLOBIN; MCF10A; MCF7;
D O I：
10.2217/nnm-2018-0335
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aim: The aim of this paper is to provide images of the universal cancer Raman biomarkers based on lipidomic, proteomic, glycomic profiles and nanomechanical properties. Materials & methods: Biochemical mapping and nanomechanical properties (topography, stiffness and adhesion) of human breast and brain for normal and cancer tissues and cell culture line U87 MG of glioblastoma were obtained using Raman imaging combined with atomic force microscopy (AFM) and fluorescence microscopy. Results & conclusion: Detailed analysis of breast ductal carcinoma in situ, and astrocytoma brain tissues as well as cells of glioblastoma U87 MG showed that Raman scattering generates images as accurately as histology hematoxylin and eosin stain used in clinical practice with additional advantage of biochemical information. Combination of AFM maps and Raman images allows to correlate mechanical properties with biochemical composition of cells. [GRAPHICS] .
引用
收藏
页码：1873 / 1888
页数：16
相关论文
共 36 条
[1]   Apical-basal polarity of epithelial cells imaged by Raman microscopy and Raman imaging: Capabilities and challenges for cancer research [J].
Abramczyk, H. ;
Brozek-Pluska, B. .
JOURNAL OF MOLECULAR LIQUIDS, 2017, 245 :52-61
[2]   The biochemical, nanomechanical and chemometric signatures of brain cancer [J].
Abramczyk, Halina ;
Imiela, Anna .
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2018, 188 :8-19
[3]   Epigenetic changes in cancer by Raman imaging, fluorescence imaging, AFM and scanning near-field optical microscopy (SNOM). Acetylation in normal and human cancer breast cells MCF10A, MCF7 and MDA-MB-231 [J].
Abramczyk, Halina ;
Surmacki, Jakub ;
Kopec, Monika ;
Olejnik, Alicja Klaudia ;
Kaufman-Szymczyk, Agnieszka ;
Fabianowska-Majewska, Krystyna .
ANALYST, 2016, 141 (19) :5646-5658
[4]   New look inside human breast ducts with Raman imaging. Raman candidates as diagnostic markers for breast cancer prognosis: Mammaglobin, palmitic acid and sphingomyelin [J].
Abramczyk, Halina ;
Brozek-Pluska, Beata .
ANALYTICA CHIMICA ACTA, 2016, 909 :91-100
[5]   The role of lipid droplets and adipocytes in cancer. Raman imaging of cell cultures: MCF10A, MCF7, and MDA-MB-231 compared to adipocytes in cancerous human breast tissue [J].
Abramczyk, Halina ;
Surmacki, Jakub ;
Kopec, Monika ;
Olejnik, Alicja Klaudia ;
Lubecka-Pietruszewska, Katarzyna ;
Fabianowska-Majewska, Krystyna .
ANALYST, 2015, 140 (07) :2224-2235
[6]   Raman Imaging in Biochemical and Biomedical Applications. Diagnosis and Treatment of Breast Cancer [J].
Abramczyk, Halina ;
Brozek-Pluska, Beata .
CHEMICAL REVIEWS, 2013, 113 (08) :5766-5781
[7]   Raman 'optical biopsy' of human breast cancer [J].
Abramczyk, Halina ;
Brozek-Pluska, Beata ;
Surmacki, Jakub ;
Jablonska-Gajewicz, Joanna ;
Kordek, Radzislaw .
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 2012, 108 (1-2) :74-81
[8]  
Al Joudi FS, 2014, INDIAN J MED RES, V139, P675
[9]   Lipid biology of breast cancer [J].
Baumann, Jan ;
Sevinsky, Christopher ;
Conklin, Douglas S. .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2013, 1831 (10) :1509-1517
[10]   Lipid metabolic reprogramming in cancer cells [J].
Beloribi-Djefaflia, S. ;
Vasseur, S. ;
Guillaumond, F. .
ONCOGENESIS, 2016, 5 :e189-e189
1234