ACTIVATION OF TRANSIENT RECEPTOR POTENTIAL ANKYRIN 1 BY EUGENOL

被引：46

作者：

Chung, G. ^{[1
,2
,3
]}

Im, S. T. ^{[1
,2
]}

Kim, Y. H. ^{[1
,2
,3
]}

Jung, S. J. ^{[3
,5
]}

Rhyu, M. -R. ^{[6
]}

Oh, S. B. ^{[1
,2
,3
,4
]}

机构：

[1] Seoul Natl Univ, Sch Dent, Dent Res Inst, Seoul 110744, South Korea

[2] Seoul Natl Univ, Sch Dent, Dept Neurobiol & Physiol, Seoul 110744, South Korea

[3] Seoul Natl Univ, Pain Cognit Funct Res Ctr, Seoul 110744, South Korea

[4] Seoul Natl Univ, Coll Nat Sci, Dept Brain & Cognit Sci, Seoul 110744, South Korea

[5] Hanyang Univ, Coll Med, Dept Physiol, Seoul 133791, South Korea

[6] Korea Food Res Inst, Div Metab & Funct Res, Songnam, South Korea

来源：

NEUROSCIENCE | 2014年 / 261卷

基金：

新加坡国家研究基金会;

关键词：

TRPA1; eugenol; trigeminal ganglion; AITC; pain; analgesia; SUBSTANTIA-GELATINOSA NEURONS; TRIGEMINAL GANGLION NEURONS; DENTAL AFFERENT NEURONS; GATED SODIUM CURRENTS; CHANNEL TRPA1; CALCIUM CURRENTS; SPINAL-CORD; CAPSAICIN; EXPRESSION; PAIN;

D O I：

10.1016/j.neuroscience.2013.12.047

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Eugenol is a bioactive plant extract used as an analgesic agent in dentistry. The structural similarity of eugenol to cinnamaldehyde, an active ligand for transient receptor potential ankyrin 1 (TRPA1), suggests that eugenol might produce its effect via TRPA1, in addition to TRPV1 as we reported previously. In this study, we investigated the effect of eugenol on TRPA1, by fura-2-based calcium imaging and patch clamp recording in trigeminal ganglion neurons and in a heterologous expression system. As the result, eugenol induced robust calcium responses in rat trigeminal ganglion neurons that responded to a specific TRPA1 agonist, allyl isothiocyanate (AITC), and not to capsaicin. Capsazepine, a TRPV1 antagonist failed to inhibit eugenol-induced calcium responses in AITC-responding neurons. In addition, eugenol response was observed in trigeminal ganglion neurons from TRPV1 knockout mice and human embryonic kidney 293 cell lines that express human TRPA1, which was inhibited by TRPA1-specific antagonist HC-030031. Eugenol-evoked TRPA1 single channel activity and eugenol-induced TRPA1 currents were dose-dependent with EC50 of 261.5 mu M. In summary, these results demonstrate that the activation of TRPA1 might account for another molecular mechanism underlying the pharmacological action of eugenol. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.

引用

页码：153 / 160

页数：8