A guide to large-scale RNA sample preparation

被引：66

作者：

Baronti, Lorenzo ^{[1
]}

Karlsson, Hampus ^{[1
]}

Marusic, Maja ^{[1
]}

Petzold, Katja ^{[1
]}

机构：

[1] Karolinska Inst, Dept Med Biochem & Biophys, Scheeles Vag 2, S-17177 Stockholm, Sweden

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2018年 / 410卷 / 14期

关键词：

RNA; Sample preparation; In vitro transcription; Chemical synthesis; Preparative high-performance liquid chromatography; Structural biology; RECOMBINANT RNA; IN-VITRO; SINGLE-MOLECULE; EXTRACELLULAR PRODUCTION; SECONDARY STRUCTURE; CHEMICAL-SYNTHESIS; PURIFICATION; CHROMATOGRAPHY; RIBOSOME; VIVO;

D O I：

10.1007/s00216-018-0943-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA is becoming more important as an increasing number of functions, both regulatory and enzymatic, are being discovered on a daily basis. As the RNA boom has just begun, most techniques are still in development and changes occur frequently. To understand RNA functions, revealing the structure of RNA is of utmost importance, which requires sample preparation. We review the latest methods to produce and purify a variation of RNA molecules for different purposes with the main focus on structural biology and biophysics. We present a guide aimed at identifying the most suitable method for your RNA and your biological question and highlighting the advantages of different methods.

引用

页码：3239 / 3252

页数：14

共 129 条

[1] RNA synthesis and purification for structural studies [J].