Preparation and characterization of monoclonal antibodies to the cholera toxin

被引：2
|
作者：
Petrova, E. E. [1 ]
Komaleva, R. L. [1 ]
Lakhtina, O. E. [1 ]
Samokhvalova, L. V. [1 ]
Kalinina, N. A. [1 ]
Shoshina, N. S. [1 ]
Rubina, A. Yu. [2 ]
Filippova, M. A. [2 ]
Vertiev, Yu. V. [3 ]
Valyakina, T. I. [1 ]
Grishin, Ye. V. [1 ]
机构：
[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia
[2] Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow, Russia
[3] Russian Acad Med Sci, Gamaleya Res Inst Epidemiol & Microbiol, Moscow, Russia
来源：
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2009年 / 35卷 / 03期
关键词：
enzyme immunoassay; sandwich assay; biological microchip; B subunit of cholera toxin; cholera toxin; monoclonal antibodies; IMMUNOASSAY; ENTEROTOXINS; MICROCHIPS; PROTEINS;
D O I：
10.1134/S1068162009030078
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Monoclonal antibodies to cholera toxin were obtained. They do not cross-react with the termolabile toxin (LT) of Escherichia coli, ricin, diphtherial toxin, staphylococcus enterotoxins of SEA, SEB, SEI, SEG, or the lethal factor and protective antigen of the anthrax toxin. Pairs of antibodies for the quantitative measurement of the cholera toxin in sandwich enzyme immunoassay (EIA) were selected. The detection limit of the toxin is 0.2 ng/ml for plate EIA and 0.44 ng/ml for microchip EIA. The presence of milk, broth, or surface water in the toxin samples does not reduce the sensitivity of EIA.
引用
收藏
页码：326 / 334
页数：9
相关论文
共 50 条
  • [41] Production and characterization of monoclonal antibodies against Campylobacter fetus subsp venerealis
    Alves, Telma M.
    Heneine, Luiz G. D.
    Araujo, Barbara S.
    Silva, Luciana M.
    Campos, Patricia C.
    Hermogenes, Marcia S.
    Lage, Andrey P.
    PESQUISA VETERINARIA BRASILEIRA, 2012, 32 (07): : 640 - 644
  • [42] Production and characterization of monoclonal antibodies for immunoassay of the lung cancer marker proGRP
    Nordlund, Marianne S.
    Fermer, Christian
    Nilsson, Olle
    Warren, David J.
    Paus, Elisabeth
    TUMOR BIOLOGY, 2007, 28 (02) : 100 - 110
  • [43] Production and Characterization of Monoclonal Antibodies Specific to Pangasius Catfish, Basa, and Tra
    Gajewski, K. G.
    Chen, Y. -T.
    Hsieh, Y. -H. P.
    JOURNAL OF FOOD SCIENCE, 2009, 74 (03) : C241 - C247
  • [44] PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO N-7-PHENYLGUANINE
    SCHELL, C
    VERKOYEN, C
    KREWET, E
    MULLER, G
    NORPOTH, K
    JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 1993, 119 (04) : 221 - 226
  • [45] PREPARATION OF MONOCLONAL-ANTIBODIES TO THE THROMBIN HIRUDIN COMPLEX
    SCHLAEPPI, JM
    THROMBOSIS RESEARCH, 1991, 62 (05) : 459 - 470
  • [46] Preparation of monoclonal antibodies reactive to a hallucinogenic drug, psilocin
    Yamaguchi, M
    Saito, T
    Horiguchi, Y
    Ogawa, K
    Tsuchiya, Y
    Hishinuma, K
    Chikuma, T
    Makino, Y
    Hojo, H
    JOURNAL OF HEALTH SCIENCE, 2004, 50 (06) : 600 - 604
  • [47] SPERM PREPARATION AFFECTS REACTIVITY OF MONOCLONAL-ANTIBODIES
    GUPTA, SK
    FULGHAM, DL
    ALEXANDER, NJ
    JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 1990, 18 (02) : 187 - 197
  • [48] Preparation of diagnostic monoclonal antibodies against two potyviruses
    Subr, Z
    Matisová, J
    ACTA VIROLOGICA, 1999, 43 (04) : 255 - 257
  • [49] Characterization of carp thrombocytes with specific monoclonal antibodies
    Rombout, JHWM
    vanDiepen, JCEK
    Emmer, PM
    TaverneThiele, JJ
    Taverne, N
    JOURNAL OF FISH BIOLOGY, 1996, 49 (03) : 521 - 531
  • [50] Development and Characterization of Monoclonal Antibodies for the Mycotoxin Citreoviridin
    Maragos, Chris M.
    Uchiyama, Yosuke
    Kobayashi, Naoki
    Kominato, Fumichika
    Sugita-Konishi, Yoshiko
    TOXINS, 2019, 11 (11)
12345