Preparation and characterization of monoclonal antibodies to the cholera toxin

被引：2
|
作者：
Petrova, E. E. [1 ]
Komaleva, R. L. [1 ]
Lakhtina, O. E. [1 ]
Samokhvalova, L. V. [1 ]
Kalinina, N. A. [1 ]
Shoshina, N. S. [1 ]
Rubina, A. Yu. [2 ]
Filippova, M. A. [2 ]
Vertiev, Yu. V. [3 ]
Valyakina, T. I. [1 ]
Grishin, Ye. V. [1 ]
机构：
[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia
[2] Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow, Russia
[3] Russian Acad Med Sci, Gamaleya Res Inst Epidemiol & Microbiol, Moscow, Russia
来源：
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2009年 / 35卷 / 03期
关键词：
enzyme immunoassay; sandwich assay; biological microchip; B subunit of cholera toxin; cholera toxin; monoclonal antibodies; IMMUNOASSAY; ENTEROTOXINS; MICROCHIPS; PROTEINS;
D O I：
10.1134/S1068162009030078
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Monoclonal antibodies to cholera toxin were obtained. They do not cross-react with the termolabile toxin (LT) of Escherichia coli, ricin, diphtherial toxin, staphylococcus enterotoxins of SEA, SEB, SEI, SEG, or the lethal factor and protective antigen of the anthrax toxin. Pairs of antibodies for the quantitative measurement of the cholera toxin in sandwich enzyme immunoassay (EIA) were selected. The detection limit of the toxin is 0.2 ng/ml for plate EIA and 0.44 ng/ml for microchip EIA. The presence of milk, broth, or surface water in the toxin samples does not reduce the sensitivity of EIA.
引用
收藏
页码：326 / 334
页数：9
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