Bilirubin binding properties of pigeon serum albumin and its comparison with human serum albumin

被引：11

作者：

Khan, MA ^{[1
]}

Kumar, Y ^{[1
]}

Tayyab, S ^{[1
]}

机构：

[1] Aligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Aligarh 202002, Uttar Pradesh, India

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2002年 / 30卷 / 3-4期

关键词：

pigeon serum albumin; human serum albumin; bilirubin; photoirradiation;

D O I：

10.1016/S0141-8130(02)00017-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Binding of bilirubin (BR) to pigeon serum albumin (PgSA) was studied by absorption, fluorescence and CD spectroscopy and results were compared with those obtained with human serum albumin (HSA). PgSA was found to be structurally similar to HSA as judged by near- and far-UV CD spectra. However, PgSA lacks tryptophan. Binding of BR to PgSA showed relatively weaker interaction compared to HSA in terms of binding affinity, induced red shift in the absorption spectrum of BR and CD spectral characteristics of BR-albumin complexes. Photoirradiation results of BR-albumin complexes also showed PgSA-bound BR more labile compared to HSA-bound BR. (C) 2002 Published by Elsevier Science B.V.

引用

页码：171 / 178

页数：8

共 30 条

[1] Use of fluorescence enhancement technique to study bilirubin-albumin interaction [J].