Analysis of genetic diversity in Cider arietinum L using random amplified polymorphic DNA markers

被引：7

作者：

Singh, R ^{[1
]}

Prasad, CD ^{[1
]}

Singhal, V ^{[1
]}

Randhawa, GJ ^{[1
]}

机构：

[1] Natl Bur Plant Genet Resources, Natl Res Ctr DNA Fingerprinting, New Delhi 110012, India

来源：

JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY | 2002年 / 11卷 / 02期

关键词：

Cicer arietinum; RAPD; genetic diversity;

D O I：

10.1007/BF03263145

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PCR-based random amplified polymorphic DNA (RAPD) markers were employed to assess genetic diversity in 23 chickpea genotypes. Forty of the 100 random primers screened revealed polymorphism among the genotypes. Most of the primers revealed single polymorphic band, and only 14.12% of the products were polymorphic. Estimates of genetic similarity based on Jaccard's coefficient ranged from 0.92 to 0.99, indicating narrow genetic variability among the genotypes based on RAPD markers. The 23 chickpea genotypes formed two major clusters in the dendrogram. The low RAPD polymorphism among chickpea genotypes suggests that more number of polymorphic primers need to be analysed to determine genetic relationships. It was observed that RAPD analysis employing 30 polymorphic primers could provide better estimates of genetic relationships in chickpea.

引用

页码：109 / 112

页数：4

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