Role of eIF3a in regulating cell cycle progression

被引：77

作者：

Dong, Zizheng ^{[1
]}

Liu, Zhaoqian ^{[1
]}

Cui, Ping ^{[1
]}

Pincheira, Roxana ^{[1
]}

Yang, Youyun ^{[1
]}

Liu, Jianguo ^{[1
]}

Zhang, Jian-Ting ^{[1
]}

机构：

[1] Indiana Univ, Dept Pharmacol & Toxicol, Walther Canc Inst, Sch Med,IU Simon Canc Ctr,Walther Oncol Ctr, Indianapolis, IN 46202 USA

来源：

EXPERIMENTAL CELL RESEARCH | 2009年 / 315卷 / 11期

关键词：

eIF3a; eIF3; p170; Cell cycle; Modulators; Translational control; INITIATION-FACTOR; 3; RIBONUCLEOTIDE REDUCTASE M2; TRANSLATION; EXPRESSION; PROTEIN; ASSOCIATION; PHASE; P170; P150; IDENTIFICATION;

D O I：

10.1016/j.yexcr.2009.03.009

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Translational control is an essential process in regulation of gene expression, which occurs at the initiation step performed by a number of translation initiation factor complexes. eIF3a (eIF3 p170) is the largest subunit of the eIF3 complex. eIF3a has been suggested to play roles in regulating translation of a subset of mRNAs and in regulating cell cycle progression and cell proliferation. In this study, we examined the expression profile of eIF3a in cell cycle and its role in cell cycle progression. We found that eIF3a expression oscillated with cell cycle and peaked in S phase. Reducing eIF3a expression also reduced cell proliferation rate by elongating cell cycle but did not change the cell cycle distribution. However, eIF3a appears to play an important role in cellular responses to external cell cycle modulators likely by affecting synthesis of target proteins of these modulators. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：1889 / 1894

页数：6

共 29 条

[1] 20S proteasome differentially alters translation of different mRNAs via the cleavage of elF4F and elF3 [J].

Baugh, JM ;

Pilipenko, EV .

MOLECULAR CELL, 2004, 16 (04) :575-586

[2] PURIFICATION AND CHARACTERIZATION OF INITIATION-FACTOR IF-E3 FROM RABBIT RETICULOCYTES [J].

BENNE, R ;

HERSHEY, JWB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (09) :3005-3009

[3] AN S-PHASE SPECIFIC RELEASE FROM A TRANSCRIPTIONAL BLOCK REGULATES THE EXPRESSION OF MOUSE RIBONUCLEOTIDE REDUCTASE R2-SUBUNIT [J].

BJORKLUND, S ;

SKOGMAN, E ;

THELANDER, L .

EMBO JOURNAL, 1992, 11 (13) :4953-4959

[4]

BJORKLUND S, 1990, BIOCHEMISTRY-US, V29, P5452

[5] Characterization of cDNAs encoding the p44 and p35 subunits of human translation initiation factor eIF3 [J].

Block, KL ;

Vornlocher, HP ;

Hershey, JWB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (48) :31901-31908

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7] Functional analysis of the interaction between HCV 5′UTR and putative subunits of eukaryotic translation initiation factor elF3 [J].

Buratti, E ;

Tisminetzky, S ;

Zotti, M ;

Baralle, FE .

NUCLEIC ACIDS RESEARCH, 1998, 26 (13) :3179-3187

[8] Biochemical characterization of mammalian translation initiation factor 3 (eIF3) -: Molecular cloning reveals that p110 subunit is the mammalian homologue of Saccharomyces cerevisiae protein Prt1 [J].

Chaudhuri, J ;

Chakrabarti, A ;

Maitra, U .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (49) :30975-30983

[9]

Chen GP, 1999, INT J CANCER, V84, P95, DOI 10.1002/(SICI)1097-0215(19990420)84:2<95::AID-IJC1>3.0.CO

[10]

2-N

← 1 2 3 →