Sensitive detection of Porcine circovirus-2 by droplet digital polymerase chain reaction

被引：33

作者：

Zhao, Shan ^{[1
]}

Lin, Hua ^{[3
]}

Chen, Shijie ^{[3
]}

Yang, Miao ^{[3
]}

Yan, Qigui ^{[1
,2
]}

Wen, Caifang ^{[1
]}

Hao, Zhongxiang ^{[1
]}

Yan, Yubao ^{[3
]}

Sun, Yingjie ^{[3
]}

Hu, Juan ^{[3
]}

Chen, Zhenrong ^{[1
]}

Xi, Lixin ^{[1
]}

机构：

[1] Sichuan Agr Univ, Coll Vet Med, Chengdu 611130, Peoples R China

[2] Sichuan Agr Univ, Key Lab Anim Dis & Human Hlth Sichuan Prov, Chengdu 611130, Peoples R China

[3] Sichuan Entry Exit Inspect & Quarantine Bur, Chengdu, Peoples R China

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2015年 / 27卷 / 06期

关键词：

Detection; droplet digital PCR; Porcine circovirus-2; TaqMan real-time PCR; REAL-TIME PCR; PORCINE CIRCOVIRUS TYPE-2; DNA COPY NUMBER; QUANTITATIVE DETECTION; QUANTIFICATION; SAMPLES; ASSAY; PIGS;

D O I：

10.1177/1040638715608358

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Sensitive detection of Porcine circovirus-2 (PCV-2) is very important for surveillance of postweaning multisystemic wasting syndrome. Droplet digital polymerase chain reaction (ddPCR) is novel PCR method that can achieve high precision. Our study aimed to develop a sensitive assay utilizing ddPCR to detect PCV-2. Specificity of the assay was confirmed by the failure of amplification of DNA of other relevant viruses. The detection limit for ddPCR was 25 copies/L, a 4-fold greater sensitivity than TaqMan real-time PCR. Both methods showed a high degree of linearity (R-2 = similar to 1), although TaqMan real-time PCR showed less sensitivity than ddPCR for clinical detection. Our findings indicate that ddPCR might represent a promising platform for detecting PCV-2 viral loads.

引用

页码：784 / 788

页数：5

共 16 条

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