In-column versus on-column fluorescence detection in capillary electrochromatography

被引：51

作者：

Rebscher, H ^{[1
]}

Pyell, U ^{[1
]}

机构：

[1] UNIV MARBURG,FACHBEREICH CHEM,D-35032 MARBURG,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 737卷 / 02期

关键词：

detection; LC; fluorescence detection; electrochromatography;

D O I：

10.1016/0021-9673(95)01378-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary electrochromatography (CEC) is a technique that uses electroosmotic flow for the propulsion of the mobile phase in liquid chromatography. Employing packed fused-silica capillaries, fluorescence detection is possible in a packed section of the separation capillary (in-column detection) or in an emptied section (on-column detection). These two detection modes were compared with each other with respect to detection limit, precision and instrumental band-broadening effects. By monitoring plug-like solute zones it is shown that with in-column detection the signal noise is dependent on the signal height, whereas with on-column detection the signal noise is independent of the signal height.

引用

页码：171 / 180

页数：10

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