Functional properties and [Ca2+]i metabolism of creatine kinase -: KO mice myocardium

被引：6

作者：

Bonz, AW ^{[1
]}

Kniesch, S ^{[1
]}

Hofmann, U ^{[1
]}

Küllmer, S ^{[1
]}

Bauer, L ^{[1
]}

Wagner, H ^{[1
]}

Ertl, G ^{[1
]}

Spindler, M ^{[1
]}

机构：

[1] Univ Wurzburg, Dept Cardiol, D-97080 Wurzburg, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 298卷 / 01期

关键词：

creatine kinase; transgenic mice; calcium; intact fibers; myocardium; energy metabolism; force-frequency relationship; post-rest behavior;

D O I：

10.1016/S0006-291X(02)02402-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

One major function of the creatine kinase system is to maintain energy demand of myofibrillar contraction processes. Loss of the CK-system led to adaptations in skeletal muscle. To analyze the impact on myocardial function contractile parameters and intracellular calcium metabolism of transgenic mice lacking mitochondrial CK (ScCKmit(-/-)) alone or both mitochondrial and cytoplasmic SICK (CK-/-) were investigated compared to wild type at various workload conditions using isolated intact muscle fibers. Force development at baseline conditions, force-frequency relationship (60-600/min), and rapid frequency switch (60-600/min) were unaltered in myocardium of transgenic mice compared to wild type. Intracellular calcium metabolism revealed unchanged amplitude of the intracellular calcium transients (ICT), refilling of the sarcoplasmic reticulum (calcium reuptake, post-rest behavior) in the ScCKmit(-/-) and CK-/- mice. The results demonstrate the effectiveness of myocardial energy-recruiting compensatory mechanisms at baseline as well as under stress conditions in CK depleted myocardium of transgenic mice. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：163 / 168

页数：6

共 22 条

[1] Maintained coupling of oxidative phosphorylation to creatine kinase activity in sarcomeric mitochondrial creatine kinase-deficient mice [J].