Cytochrome P450 expression in rat ocular tissues and its induction by phenobarbital

被引：6

作者：

Tanaka, H ^{[1
]}

Hirayama, I ^{[1
]}

Takehana, M ^{[1
]}

Kobayashi, S ^{[1
]}

Tamura, H ^{[1
]}

机构：

[1] Kyoritsu Coll Pharmaceut Sci, Minato Ku, Tokyo 1058512, Japan

来源：

JOURNAL OF HEALTH SCIENCE | 2002年 / 48卷 / 04期

关键词：

cytochrome P450; ocular tissue; lens;

D O I：

10.1248/jhs.48.346

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Composition and inducibility of cytochrome P450 (CYP) in ocular tissues were investigated using reverse transcription-polymerise chain reaction (RT-PCR) and immunoblot techniques. Composition of ocular CYPs is more restricted than that of hepatic forms. High levels of CYP2B1/2 and reduced levels of CYP2C11 expression were detected in rat ocular tissues. Phenobarbital induced CYP2B1/2 expression in the lens but not in the rest of the eye, whereas CYP2C11 induction was observed in both regions. In addition, CYP2B1/2 proteins were found to be prominent in the retina. Our data indicate eye-specific regulation of CYP expression.

引用

页码：346 / 349

页数：4

共 10 条

[1] Analysis of cytochrome P450 and phase II conjugating enzyme expression in adult male rat hepatocytes
Davila, JC
Morris, DL
[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 1999, 35 (03) : 120 - 130
[2] EXTRAHEPATIC METABOLISM OF DRUGS IN HUMANS
KRISHNA, DR
KLOTZ, U
[J]. CLINICAL PHARMACOKINETICS, 1994, 26 (02) : 144 - 160
[3] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[4] Mashima Y, 2001, INVEST OPHTH VIS SCI, V42, P2211
[5] THE P450 SUPERFAMILY - UPDATE ON NEW SEQUENCES, GENE-MAPPING, ACCESSION NUMBERS, EARLY TRIVIAL NAMES OF ENZYMES, AND NOMENCLATURE
NELSON, DR
KAMATAKI, T
WAXMAN, DJ
GUENGERICH, FP
ESTABROOK, RW
FEYEREISEN, R
GONZALEZ, FJ
COON, MJ
GUNSALUS, IC
GOTOH, O
OKUDA, K
NEBERT, DW
[J]. DNA AND CELL BIOLOGY, 1993, 12 (01) : 1 - 51
[6] Cytotoxic metabolite of acetaminophen, N-acetyl-p-benzoquinone imine, produces cataract in DBA2 mice
Qian, W
Amin, RH
Shichi, H
[J]. JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS, 1999, 15 (06) : 537 - 545
[7] CYTOCHROME-P450, DRUG-METABOLIZING-ENZYMES AND ARACHIDONIC-ACID METABOLISM IN BOVINE OCULAR-TISSUES
SCHWARTZMAN, ML
MASFERRER, J
DUNN, MW
MCGIFF, JC
ABRAHAM, NG
[J]. CURRENT EYE RESEARCH, 1987, 6 (04) : 623 - 630
[8] IMMUNOCYTOCHEMICAL STUDY OF PHENOBARBITAL-INDUCIBLE AND 3-METHYLCHOLANTHRENE-INDUCIBLE CYTOCHROME P450 ISOZYMES IN PRIMARY CULTURES OF PORCINE CILIARY EPITHELIUM
SHICHI, H
MAHALAK, SM
SAKAMOTO, S
SUGIYAMA, T
[J]. CURRENT EYE RESEARCH, 1991, 10 (08) : 779 - 788
[9] Effect of phenobarbital on intralobular expression of CYP2B1/2 in livers of rats - Difference in the expression between single and repetitive administrations
Watanabe, J
Mondo, H
Takamori, Y
Takeda, K
Kanamura, S
[J]. BIOCHEMICAL PHARMACOLOGY, 2000, 60 (02) : 285 - 291
[10] Histocytological study on the possible mechanism of acetaminophen cataractogenesis in mouse eye
Zhao, C
Shichi, H
[J]. EXPERIMENTAL AND MOLECULAR PATHOLOGY, 1995, 63 (02) : 118 - 128

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