Protein production: feeding the crystallographers and NMR spectroscopists

被引：126

作者：

Edwards, AM

Arrowsmith, CH

Christendat, D

Dharamsi, A

Friesen, JD

Greenblatt, JF

Vedadi, M

机构：

[1] Ontario Canc Inst, Toronto, ON, Canada

[2] Univ Toronto, Banting & Best Dept Med Res, Toronto, ON, Canada

[3] Integrat Proteom, Toronto, ON, Canada

来源：

NATURE STRUCTURAL BIOLOGY | 2000年 / 7卷 / Suppl 11期

关键词：

D O I：

10.1038/80751

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein purification efforts for structural genomics will focus on automation for the readily-expressed proteins, and process development for the more difficult ones, such as membrane proteins. Thousands of proteins are expected to be produced in the next few years. The purified proteins will be valuable reagents for the entire research community.

引用

收藏

页码：970 / 972

页数：3

相关论文

共 15 条

[1] OVEREXPRESSION, PURIFICATION, AND CRYSTALLIZATION OF THE DNA-BINDING AND DIMERIZATION DOMAINS OF THE EPSTEIN-BARR-VIRUS NUCLEAR ANTIGEN-1 [J].

BARWELL, JA ;

BOCHKAREV, A ;

PFUETZNER, RA ;

TONG, H ;

YANG, DSC ;

FRAPPIER, L ;

EDWARDS, AM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (35) :20556-20559

[2]

BAX A, 1991, CIBA F SYMP, V161, P108

[3] The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain [J].

Bochkareva, E ;

Frappier, L ;

Edwards, AM ;

Bochkarev, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (07) :3932-3936

[4]

Christendat D, 2000, NAT STRUCT BIOL, V7, P903

[5]

CHRISTENDAT D, 2000, IN PRESS PROGR BIOPH

[6] PROBING THE SOLUTION STRUCTURE OF THE DNA-BINDING PROTEIN MAX BY A COMBINATION OF PROTEOLYSIS AND MASS-SPECTROMETRY [J].

COHEN, SL ;

FERREDAMARE, AR ;

BURLEY, SK ;

CHAIT, BT .

PROTEIN SCIENCE, 1995, 4 (06) :1088-1099

[7] NMR structure and mutagenesis of the FADD (Mort1) death-effector domain [J].

Eberstadt, M ;

Huang, BH ;

Chen, ZH ;

Meadows, RP ;

Ng, SC ;

Zheng, LX ;

Lenardo, MJ ;

Fesik, SW .

NATURE, 1998, 392 (6679) :941-945

[8] A PRACTICAL METHOD FOR UNIFORM ISOTOPIC LABELING OF RECOMBINANT PROTEINS IN MAMMALIAN-CELLS [J].

HANSEN, AP ;

PETROS, AM ;

MAZAR, AP ;

PEDERSON, TM ;

RUETER, A ;

FESIK, SW .

BIOCHEMISTRY, 1992, 31 (51) :12713-12718

[9] THE TREATMENT OF METASTATIC BREAST-CANCER WITH ADJUVANT SYSTEMIC THERAPY [J].

HENDERSON, IC .

ANNALS OF ONCOLOGY, 1990, 1 (01) :9-10

[10] Cell-free production and stable-isotope labeling of milligram quantities of proteins [J].

Kigawa, T ;

Yabuki, T ;

Yoshida, Y ;

Tsutsui, M ;

Ito, Y ;

Shibata, T ;

Yokoyama, S .

FEBS LETTERS, 1999, 442 (01) :15-19