Influence of nitric oxide on microcirculation in pancreatic ischemia/reperfusion injury: an intravital microscopic study

被引:10
作者
Obermaier, R
von Dobschuetz, E
Muhs, O
Keck, T
Drognitz, O
Jonas, L
Schareck, W
Hopt, UT
Benz, S
机构
[1] Univ Freiburg, Dept Surg, D-79106 Freiburg, Germany
[2] Univ Rostock, Dept Gen Vasc Thorac & Transplantat Surg, Rostock, Germany
[3] Univ Rostock, Dept Pathol, Rostock, Germany
关键词
nitric oxide; ischemia/reperfusion; pancreas; intravital microscopy; microcirculation;
D O I
10.1007/s00147-004-0702-y
中图分类号
R61 [外科手术学];
学科分类号
摘要
Recently, protective effects of nitric oxide donors in pancreatic ischemia/reperfusion (IRI) injury have been described. Their role in post-ischemic microcirculation was previously not investigated. Ischemia reperfusion was induced in an isolated pancreatic tail segment in situ. Animals were randomized to four experimental groups (n=7 animals/group), the control group (CO) received saline as placebo. Treatment groups received either sodium nitroprusside (SN) 5 min before until 2 h after reperfusion, L-arginine (LA) 30 min before reperfusion until 2 h after reperfusion or sodium nitroprusside and L-arginine (SNLA) together. After induction of ischemia (2 h) post-ischemic microcirculation was observed for 2 h by intravital-fluorescence microscopy. Functional-capillary density (FCD), leukocyte adherence in post-capillary venules (LAV) and histological damage were analysed. After reperfusion FCD decreased in all groups (P<0.05). FCD was significantly restored in all groups with administration of nitric oxide donors after reperfusion (P<0.05) as compared to CO without significant difference between the individual nitric oxide donor groups. Leukocyte adherence was significantly increased 1 h and 2 h after reperfusion (P<0.001) as compared to baseline, which was lower in all nitric oxide donor groups. Histological damage in the pancreatic tail-segment was significantly reduced in nitric oxide donor groups (P<0.01). Administration of nitric oxide donors might be useful in ischemia-reperfusion injury of the pancreas by its protective effect on microcirculation and inflammatory reaction.
引用
收藏
页码:208 / 214
页数:7
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