Effect of nanostructures on anchoring stem cell-derived neural tissue to artificial surfaces

被引：10

作者：

Berces, Z. ^{[1
,2
]}

Pomothy, J. ^{[3
]}

Horvath, A. Cs ^{[1
,2
,4
]}

Kohidi, T. ^{[3
]}

Benyei, E. ^{[3
]}

Fekete, Z. ^{[1
,2
]}

Madarasz, E. ^{[3
]}

Pongracz, A. ^{[1
,2
]}

机构：

[1] Pazmany Peter Catholic Univ, Fac Informat Technol & Bion, Res Grp Implantable Microsyst, 50-A Prater St, H-1083 Budapest, Hungary

[2] Hungarian Acad Sci, Inst Tech Phys & Mateial Sci, Res Ctr Nat Sci, 29-33 Konkoly Thege St, H-1121 Budapest, Hungary

[3] HAS, Lab Cellular & Dev Neurobiol, Inst Expt Med, 43 Szigony Str, H-1083 Budapest, Hungary

[4] Obuda Univ, Doctoral Sch Mat Sci & Technol, 96-B Becsi Str, H-1034 Budapest, Hungary

来源：

JOURNAL OF NEURAL ENGINEERING | 2018年 / 15卷 / 05期

基金：

匈牙利科学研究基金会;

关键词：

black poly-silicon; nanostructuring; cell adhesion; PROGENITOR CELLS; DIFFERENTIATION; GROWTH; BRAIN; NANOTOPOGRAPHY; NANOMATERIALS; ATTACHMENT; MICROELECTRODES; PROLIFERATION; HIPPOCAMPUS;

D O I：

10.1088/1741-2552/aad972

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Objective. Chronic application of brain implants monitoring or modulating neuronal activity are hindered by the foreign body response of the tissue. Topographical modification of implant surfaces may reduce negative tissue response by imitating the structure of the extracellular matrix and therefore affecting the attachment and behavior of neural cells. Approach. In our in vitro study, the effect of nanostructuring was investigated on two commercially used neural implant materials: silicon and platinum. The adhesion, survival and arrangement of neural stern cells (NE4C) and microglial cells (BV2) were investigated and compared to nanostructured and flat Si and Pt surfaces using cell viability studies and fluorescent microscopy image analysis. Main results. Our data indicated that neural cells established strong adhesive couplings with each other, instead of binding to the artificial surfaces. Significance. The phenomena resemble some features of in vivo separation of living tissue from the implanted artificial material, providing an in vitro model for studying immune response.

引用

页数：10

共 48 条

[41] Increased proliferation of neural progenitor cells but reduced survival of newborn cells in the contralateral hippocampus after focal cerebral ischemia in rats [J].