Antibiotic Resistance Genes and Correlations with Microbial Community and Metal Resistance Genes in Full-Scale Biogas Reactors As Revealed by Metagenomic Analysis

被引:158
|
作者
Luo, Gang [1 ]
Li, Bing [2 ]
Li, Li-Guan [3 ]
Zhang, Tong [3 ]
Angelidaki, Irini [4 ]
机构
[1] Fudan Univ, Dept Environm Sci & Engn, Shanghai Key Lab Atmospher Particle Pollut & Prev, Shanghai 200433, Peoples R China
[2] Tsinghua Univ, Grad Sch Shenzhen, Key Lab Microorganism Applicat & Risk Control She, Shenzhen 518055, Guangdong, Peoples R China
[3] Univ Hong Kong, Environm Biotechnol Lab, Hong Kong, Hong Kong, Peoples R China
[4] Tech Univ Denmark, Dept Environm Engn, DK-2800 Lyngby, Denmark
基金
中国国家自然科学基金;
关键词
BACTERIAL PATHOGENS; FOSMIDOMYCIN-CLINDAMYCIN; TETRACYCLINE RESISTANCE; ACTIVATED-SLUDGE; SEWAGE-SLUDGE; HEAVY-METAL; SOIL; COSELECTION; DIVERSITY; MACROLIDE;
D O I
10.1021/acs.est.6b05100
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Digested residues from biogas plants are often used as biofertilizers for agricultural crops cultivation. The antibiotic resistance genes (ARGs) in digested residues pose a high risk to public health due to their potential spread to the disease-causing microorganisms. and thus reduce the susceptibility of disease-causing microorganisms to antibiotics in medical treatment. A high-throughput sequencing (HTS)-based metagenomic approach was used in the present study to investigate the variations of ARGs in full-scale biogas reactors and: the correlations of ARGs with microbial communities and metal resistance genes (MRGs). The total abundance of ARGs in all the samples varied from 7 X 10(-3) to 1.08 X 10(-1) copy of ARG/copy of I6SrRNA gene, and the samples obtained from thermophilic biogas reactors had a lower total abundance of ARGs, indicating the superiority of thermophilic anaerobic digestion for ARGs removal. ARGs in all the samples were composed. of 175 ARG subtypes; however, only 7 ARG subtypes were shared by all the samples. Principal component analysis and canonical correspondence analysis clustered the samples into three groups (samples from manure -based mesophilic reactors, manure -based thermophilic reactors, and sludge -based mesophilic reactors), and substrate, temperature, and hydraulic retention time (HRT) as well as volatile fatty acids (VFAs) were identified as crucial environmental variables affecting the ARGs compositions. Procrustes analysis revealed microbial community composition was the determinant of ARGs composition in biogas reactors, and there was. also a significant correlation between ARGs composition and MRGs composition. Network analysis further revealed the co-occurrence of ARGs with specific microorganisms and MRGs.
引用
收藏
页码:4069 / 4080
页数:12
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