Restriction fragment length polymorphism (RFLP) of Salmonella organisms

被引：2

作者：

KohLuar, SI

Chew, ST

Lau, E

Chua, SB

机构：

[1] VET PUBL HLTH LAB,PRIMARY PROD DEPT,SINGAPORE 619415,SINGAPORE

[2] VET PUBL HLTH DIV,PRIMARY PROD DEPT,SINGAPORE 069110,SINGAPORE

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1996年 / 12卷 / 04期

关键词：

food pathogen; Salmonella serotypes; RFLP; 16S rDNA;

D O I：

10.1007/BF00340221

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genetic relatedness of 20 Salmonella isolates comprising 16 serotypes was analysed by restriction endonuclease digestion of the total DNA with six endonucleases individually. The rDNA fingerprints generated by EcoRI were more polymorphic, each serotype showed a unique fingerprint sharing several core (monomorphic) bands with several polymorphic bands. Eight characteristic NciI rDNA fingerprints were found. Similar rDNA RFLP patterns were observed in strains of Salmonella from different serotypes.

引用

页码：405 / 407

页数：3

共 7 条

[1] ISOLATION AND DIRECT COMPLETE NUCLEOTIDE DETERMINATION OF ENTIRE GENES - CHARACTERIZATION OF A GENE CODING FOR 16S-RIBOSOMAL RNA [J].